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H8032

Sigma-Aldrich

Hanahan′s Broth (SOB Medium)

powder microbial growth medium

Sinónimos:

Super Optimal Broth

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500 G
251,00 €

251,00 €

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500 G
251,00 €

About This Item

Código UNSPSC:
41106200
NACRES:
NA.85

251,00 €

Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad

grado

for molecular biology

Nivel de calidad

100

descripción

recommended for cultivation of recombinant E. coli.

esterilidad

non-sterile

Formulario

powder

composición

KCl, 0.186 g/L
MgSO4, 2.4 g/L
NaCl, 0.5 g/L
Tryptone, 20 g/L
Yeast extract, 5 g/L

técnicas

microbiological culture: suitable

pH

6.8-7.2(2.8% solution)

aplicaciones

food and beverages
microbiology

temp. de almacenamiento

room temp

idoneidad

nonselective for Escherichia coli
nonselective for coliforms

Categorías relacionadas

Descripción general

Hannahan’s Broth (SOB) is a microbial growth medium used for the preparation and transformation of competent cells (E. coli). Casein enzymic hydrolysate and yeast extract supply nitrogenous compounds and growth factors for recombinant Escherichia coli. Potassium chloride and sodium chloride maintain the isotonic condition. Magnesium sulfate is added to the medium as a necessary component for DNA replication.

Aplicación

Hanahan′s Broth is recommended for use in cultivation of recombinant strains of Escherichia coli. It is suitable for the preparation of competent E. coli., often with the addition of 0.4% dextrose. It has also been used for the preparation of C. necator H16 competent cells.[1] It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.

Características y beneficios

Hannahan′s Broth (SOB) powder provides:
  • Standard formulation
  • An inexpensive alternative to liquid

Reconstitución

Stir to suspend 28 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Otras notas

Casein enzymic hydrolysate and yeast extract supply the necessary nitrogenous compounds and growth factors. Potassium chloride and sodium chloride provide an isotonic environment for the cells. Magnesium sulfate aids DNA replication.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves

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Certificados de análisis (COA)

Lot/Batch Number
MKCV5945
MKCW9976
MKCS6546
MKCQ5868
MKCP4902

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Protocolos

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

Preguntas

1–2 de 2 Preguntas  

  1. Which bacterial culture medium is the best choice for my application?

    1 respuesta

    1. Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production.  The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.

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  2. What is the Department of Transportation shipping information for this product?

    1 respuesta

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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