H6254

Hyaluronidase from sheep testes

Type V, lyophilized powder, ≥1,500 units/mg solid

• Sinónimos: Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
• Número de CAS: 37326-33-3
• Comisión internacional de enzimas: 3.2.1.35 (BRENDA, IUBMB)
• Número CE: 253-464-3
• Número MDL: MFCD00131351
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• origen biológico: sheep testis
• tpo: Type V
• formulario: lyophilized powder
• actividad específica: ≥1,500 units/mg solid
• mol peso: 55 kDa
• aplicaciones: diagnostic assay manufacturing
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Hyaluronidase is present in high amount in testis as it aids fertilization of the mammalian egg. Mammalian hyaluronidase has a catalytic epidermal growth factor (EGF)-like domain and a C-terminal cysteine rich region.

Aplicación

Hyaluronidase has been used:

• as a component of enzyme mix for the isolation of sertoli and germ cells from seminiferous tubules
• in the pre-treatment of deparaffinized adenomas sections for immunohistochemistry
• in the digestion of umbilical cord blood vessel for the generation of umbilical cord stromal stem cell lines

Acciones bioquímicas o fisiológicas

Hyaluronidase from sheep testis is commercially used to depolymerise hyaluronic acid and chondroitin-sulphate in connective tissue and cartilage respectively.

These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Definición de unidad

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

Otras notas

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334
• Consejos de prudencia: P261 - P284 - P501
• Clasificaciones de peligro: Resp. Sens. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)