H5527

Hygromycin B solution from Streptomyces hygroscopicus

≥60% (HPLC), 45-60 mg/mL in H₂O, γ-irradiated

• Sinónimos: Hygromycin b, Hygrovetine
• Fórmula empírica (notación de Hill): C₂₀H₃₇N₃O₁₃
• Número de CAS: 31282-04-9
• Peso molecular: 527.52
• Beilstein: 6755837
• Número MDL: MFCD06795479
• Código UNSPSC: 51102829
• ID de la sustancia en PubChem: 24895698
• NACRES: NA.85

Propiedades

• origen biológico: Streptomyces hygroscopicus
• Nivel de calidad: 200
• esterilidad: γ-irradiated
• concentración: ≥60% (HPLC); 45-60 mg/mL in H₂O
• color: yellow-brown to orange
• espectro de actividad antibiótica: viruses
• Modo de acción: protein synthesis | interferes
• temp. de almacenamiento: 2-8°C
• cadena SMILES: CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3OC4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O
• InChI: 1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1
• Clave InChI: GRRNUXAQVGOGFE-XKIAHZFYSA-N

Descripción

Descripción general

Chemical structure: aminoglycoside

Aplicación

Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreading, as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation, and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 μg/mL, specifically at 100 μg/mL for prokaryotes, 200 μg/mL for lower eukaryotes and 150-400 μg/mL for higher eukaryotes.

Acciones bioquímicas o fisiológicas

Mode of action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation.

Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.

Precaución

Hygromycin B products should be stored as supplied at 2-8°C, and the dry solid is stable for at least 5 years if stored at 2-8°C. It is stable at 37°C for 30 days.

Nota de preparación

This product is a solution that is =60% (HPAE), 45-60 mg/mL in H₂O, and γ-irradiated .

Información de seguridad

• Pictogramas: GHS06,GHS08,GHS05
• Palabra de señalización: Danger
• Frases de peligro: H301 + H311,H318,H330,H334
• Consejos de prudencia: P260 - P280 - P284 - P301 + P310 - P305 + P351 + P338 - P310
• Clasificaciones de peligro: Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Eye Dam. 1 - Resp. Sens. 1
• Código de clase de almacenamiento: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter