H0163

Anti-Histone Deacetylase 4 (HDAC4) antibody, Mouse monoclonal

clone HDAC4-144, purified from hybridoma cell culture

• Número MDL: MFCD05665442
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: purified from hybridoma cell culture
• tipo de anticuerpo: primary antibodies
• clon: HDAC4-144, monoclonal
• Formulario: buffered aqueous solution
• mol peso: antigen ~140 kDa
• reactividad de especies: human, rat, mouse
• validación mejorada: independent; Learn more about Antibody Enhanced Validation
• concentración: ~2 mg/mL
• técnicas: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 1-2 μg/mL using total cell extracts of NIH3T3 fibroblasts cells
• isotipo: IgG2a
• Nº de acceso UniProt: P56524
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... HDAC4(9759) ; mouse ... Hdac4(208727) ; rat ... Hdac4(363287)

Descripción

Descripción general

Monoclonal Anti-Histone Deacetylase 4 (HDAC4) (mouse IgG2a isotype) is derived from the HDAC4-144 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acids of human HDAC4 with C-terminal added lysine, conjugated to KLH. HDAC4 belongs to the class II of Mammalian HDACs.

Aplicación

Anti-Histone Deacetylase 4 (HDAC4) antibody, Mouse monoclonal has been used in:

• enzyme linked immunosorbent assay (ELISA)
• immunoblotting[1]
• immunocytochemistry
• immunoprecipitation

Acciones bioquímicas o fisiológicas

Histone deacetylase (HDAC) catalyzes the deacetylation of histones. HDAC4 is implicated in transcriptional repression. It dynamically migrates between nucleus and cytoplasm through its nuclear import and export signals. Interaction of HDAC4 with 14-3-3 and myocyte enhancer factor-2 (Mef2) proteins disturbs such shuttling and thus directs HDAC4 to the cytoplasm and the nucleus, respectively.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): nwg
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable