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Merck

G9382

Sigma-Aldrich

Gelatin from bovine skin

gel strength ~225 g Bloom, Type B

Sinónimos:

Bovine gelatin

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100 G
56,70 €
500 G
209,00 €
1 KG
329,00 €

56,70 €


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100 G
56,70 €
500 G
209,00 €
1 KG
329,00 €

About This Item

Número de CAS:
Número CE:
Número MDL:
Código UNSPSC:
12352202
NACRES:
NA.26

56,70 €


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origen biológico

bovine skin

Nivel de calidad

tpo

Type B

Ensayo

≥95% protein basis (biuret)

Formulario

powder

técnicas

ELISA: suitable
cell culture | mammalian: suitable
immunocytochemistry: suitable
western blot: suitable

solubilidad

H2O: soluble 50 mg/mL

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Componentes

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.

Precaución

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Nota de preparación

This product is derived from bovine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gel strength of 225.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


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K A van der Lee et al.
Journal of lipid research, 41(1), 41-47 (2000-01-11)
Long-chain fatty acids are the most important substrates for the heart. In addition, they have been shown to affect signalling pathways and gene expression. To explore the effects of long-chain fatty acids on cardiac gene expression, neonatal rat ventricular myocytes
T H Kim et al.
Hepatology (Baltimore, Md.), 31(1), 75-82 (1999-12-29)
Partial hepatectomy triggers a variety of biological phenomena, which culminate in regeneration of the liver mass. Hepatocyte proliferation is a major feature of the regenerating liver after partial hepatectomy. Previous studies in our laboratory suggested that hepatic matrix remodeling might
Ochuko L Erukainure et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 83, 1478-1484 (2016-10-25)
The immunomodulatory potentials of the crude methanolic extract and fractions [n-hexane (Hex), n-dichloromethane (DCM), ethyl acetate (EtOAc) and n-butanol (BuOH)] of Clerodendrum volubile flowers were investigated on whole blood phagocytic oxidative burst using luminol-amplified chemiluminescence technique. They were also investigated
Isolation and maintenance of symbiotic fungi of ants in the tribe Attini (Hymenoptera: Formicidae)
Silva-Pinhati A C O, et al.
Neotropical Entomology, 34, 1-5 (2005)
Substrate specificity of thermostable alkaline protease from Bacillus sp. No. AH-101.
H Takami et al.
Bioscience, biotechnology, and biochemistry, 56(2), 333-334 (1992-02-01)

Preguntas

1–10 de 11 Preguntas  
  1. What is the bulk density of this product

    1 respuesta
    1. The bulk density of this product is not determined.

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  2. What is the molecular weight of this Gelatin?

    What is the molecular weight of this Gelatin?

    1 respuesta
    1. The molecular weight of this product has not been evaluated, However, the molecular weight is closely related to the Bloom Number. The estimated ranges are as follows:
      Bloom Number Average Molecular Mass (Da)
      50−125 (Low Bloom) 20,000−25,000
      175−225 (Medium Bloom) 40,000−50,000
      225−325 (High Bloom) 50,000−100,000

      Please see the link below to review the general datasheet for Gelatin:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/625/g9382pis.pdf

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  3. How to sterilize 2% solution?

    1 respuesta
    1. This item can be sterilized by autoclaving at 121°C, 15 psi for 30 minutes.

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  4. Which is the molecular weight?

    1 respuesta
    1. The Bloom number or gel strength of this product is ~225 g Bloom. The average molecular mass of 175-225 g Bloom (Medium Bloom) products is 40,000-50,000 Da. The average molecular mass of 225-325 g Bloom (High Bloom) products is 50,000-100,000 Da. Therefore, this product is expected to have a molecular weight of approximately 50 kDa.

      For more information please view the product data sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/187/614/g6650-et-al-pis-ms.pdf

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  5. Is this made from skin from a cow which was killed for the food chain?

    1 respuesta
    1. All information that is shared regarding source can be found on a lot-specific Certificate of Origin (CofO, COO) for this product.

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  6. What is Product G9382, Gelatin from bovine skin, soluble in?

    1 respuesta
    1. Gelatin is soluble in glycerol and acetic acid, and more soluble in hot water than in cold water.  We test the solubility of gelatin in water at 50 mg/mL (with heat).

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  7. What is the Department of Transportation shipping information for this product?

    1 respuesta
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  8. What is a Bloom number, and how does it correlate with the molecular weight?

    1 respuesta
    1. The Bloom number is indicative of gel strength. The Bloom number is the amount of pressure, in grams, required to break the surface of a 6.67% gel. The general rule of thumb in regards to an average molecular weight: High Bloom (225-325) = 50,000-100,000; Medium Bloom (175-225) = 40,000-50,000; Low Bloom (50-125) = 20,000-25,000. Product G9382 is a high Bloom preparation of approximately 225 Bloom.

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  9. What is the difference between type A and type B gelatin?

    1 respuesta
    1. Type A gelatin is derived from acid-cured tissue whereas Type B gelatin is derived from lime-cured tissue. Type A gelatin has about 78-80 millimoles of free carboxyl groups per 100 g of protein and a pI of 7.9-9.0. Type B has 100-115 millimoles of free carboxyl groups per 100 g of protein and a pI of 4.7-5.2.

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  10. What is the isoelectric point (pI) for Product G9382, Gelatin from bovine skin?

    1 respuesta
    1. The pI for Product No. G9382 is typically in the range of 4.7 - 5.0.

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1–10 de 11 Preguntas  

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