G7654
Gel Loading Solution
for NA electrophoresis, solution
Sinónimos:
DNA Gel Loading Solution, Gel Loading Buffer
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NACRES:
NA.25
UNSPSC Code:
12161703
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Servicio técnico
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Molecular Biology
Quality Segment
form
solution
concentration
6 ×
technique(s)
nucleic acid detection: suitable
storage temp.
2-8°C
General description
Gel loading solution is used as a tracking dye during electrophoresis. The dyes have a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition. Dilute 1:6 with sample before loading.
Application
Suitable for use with agarose or non-denaturing polyacrylamide gel electrophoresis (PAGE), which may be part of Northern and Southern blot hybridization procedures.
Preparation Note
Gel loading buffer contains 0.25% bromophenol blue, 0.25% xylene cyanol, and 40% sucrose.
Other Notes
Band migration can be expected as follows:
On polyacrylamide gels, xylene cyanole comigrates with approximately 450-460 bp DNA, while bromophenol blue comigrates with 15-100 bp DNA. On 0.5 – 1.4% agarose gels, xylene cyanole comigrates with 4 kb dsDNA, while bromophenol blue comigrates with 300 bp dsDNA.
On polyacrylamide gels, xylene cyanole comigrates with approximately 450-460 bp DNA, while bromophenol blue comigrates with 15-100 bp DNA. On 0.5 – 1.4% agarose gels, xylene cyanole comigrates with 4 kb dsDNA, while bromophenol blue comigrates with 300 bp dsDNA.
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Este artículo | |||
|---|---|---|---|
| description for NA electrophoresis, solution | description for NA electrophoresis | description 2X Loading dye for RNA gel electrophoresis. 500µL for 100 samples. | description Dye content ≥75 % |
| grade Molecular Biology | grade Molecular Biology | grade - | grade - |
| concentration 6 × | concentration - | concentration - | concentration - |
| Quality Level 100 | Quality Level 200 | Quality Level - | Quality Level 200 |
| form solution | form liquid | form - | form powder or crystals |
| storage temp. 2-8°C | storage temp. room temp | storage temp. 2-8°C | storage temp. room temp |
Clase de almacenamiento
12 - Non Combustible Liquids
¿Qué está pasando?
WGK 2
Punto de inflamación (°F)
Not applicable
Punto de inflamación (°C)
Not applicable
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