This product may be reconstituted in sterile water at 0.1 - 1.0 mg/mL, following centrifugation of the vial to collect the contents. Please see the link below to review the product datasheet which includes complete preparation and storage instructions:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/399/841/g5035dat.pdf
G5035
Granulocyte-Macrophage Colony-Stimulating Factor human
≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Sinónimos:
GM-CSF human
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5 μG
220,00 €
4 X 5 μG
457,00 €
220,00 €
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5 μG
220,00 €
4 X 5 μG
457,00 €
About This Item
220,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Solicitar un pedido a granel
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Nombre del producto
Granulocyte-Macrophage Colony-Stimulating Factor human, GM-CSF, recombinant, expressed in E. coli, suitable for cell culture
origen biológico
human
Nivel de calidad
recombinante
expressed in E. coli
Ensayo
≥98% (SDS-PAGE)
Formulario
lyophilized powder
potencia
≤0.1 ng/mL EC50
mol peso
14.6 kDa
envase
pkg of 4X5 μg
pkg of 5 μg
condiciones de almacenamiento
avoid repeated freeze/thaw cycles
técnicas
cell culture | mammalian: suitable
impurezas
≤1.000 EU/μg
color
white
solubilidad
water: soluble 0.010 mL, clear, colorless
Nº de acceso UniProt
temp. de almacenamiento
−20°C
Información sobre el gen
human ... CSF2(1437) , CSF2(1437)
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Descripción general
Package size based on protein content
Acciones bioquímicas o fisiológicas
Granulocyte-macrophage colony stimulating factor (GM-CSF) is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. GM-CSF stimulates colony formation from pluripotential progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages). GM-CSF is secreted as a single chain glycoprotein containing 128 amino acids for human with a conserved disulfide bond. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity.
Forma física
Lyophilized from a 0.2 μm filtered, 10 mM sodium citrate, pH 3.5 solution.
Nota de análisis
The proliferative activity of human GM-CSF is tested in culture using human TF-1 cells.[1]
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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Artículos
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What is reconstitution procedure for GM-CSF?
1 respuesta-
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Can I use Product G5035, Granulocyte-Macrophage Colony-Stimulating Factor human, on mouse cells?
1 respuesta-
There is only about 56% homology between human and mouse GM-CSF, and no cross-species activity. We would recommend to use mouse GM-CSF, Product No. G0282.
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There is no visible powder in the vial of Product G5035, Granulocyte-Macrophage Colony-Stimulating Factor human. Is the vial empty?
1 respuesta-
This product only contains 5 micrograms of protein without any carrier protein. The product is lyophilized from a small amount of solution so the product may not be visible in the vial.
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How many units are present in the vial of Product G5035, Granulocyte-Macrophage Colony-Stimulating Factor human?
1 respuesta-
The ED50 was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is less than or equal to 0.1 ng/ml, corresponding to a specific activity of greater than or equal to 1 x 107 units/mg. So the 5 microgram package size (G5035-5UG) should have at least 50,000 units per vial.
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How does the storage temperature relate to shipping conditions?
1 respuesta-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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What is the isoelectric point (pI) for Product G5035, Granulocyte-Macrophage Colony-Stimulating Factor human?
1 respuesta-
The pI is 4.8.
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What is the Department of Transportation shipping information for this product?
1 respuesta-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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