A search of the website does not show a density for product G4155. For products supplied in small quantities, it is not common for the density to be measured. However, for solutions available in larger quantities, density information is often provided. Although the density for G4155 is not stated, there is some useful information available from the website. The physical form of the product is a suspension in 50% glycerol, 5 mM Tris buffer salts, 5 mM magnesium chloride, 0.5 mM DTT, and 0.5 mM mercaptoethanol at pH 7.4.
G4155
β-Galactosidasa from Escherichia coli
aqueous glycerol suspension, ≥500 units/mg protein (biuret)
Sinónimos:
β-D-galactósido galactohidrolasa, Lactasa
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1000 UNITS
108,00 €
3000 UNITS
278,00 €
5000 UNITS
397,00 €
108,00 €
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1000 UNITS
108,00 €
3000 UNITS
278,00 €
5000 UNITS
397,00 €
About This Item
Número de CAS:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54
108,00 €
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Formulario
aqueous glycerol suspension
Nivel de calidad
actividad específica
≥500 units/mg protein (biuret)
mol peso
465 kDa
temp. de almacenamiento
−20°C
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Categorías relacionadas
Aplicación
β-Galactosidase is used in the enzymatic assays in the synthesis of imidazolo-pyrrolidinoses.
Acciones bioquímicas o fisiológicas
βLa β-galactosidasa hidroliza la lactosa en sus componentes monosacáridos, glucosa y galactosa. También cataliza la transglucosilación de la glucosa en alolactosa, el inductor de la β-galactosidasa, en un bucle de retroalimentación.
Propiedades físicas
Peso molecular del tetrámero 465 kDa (subunidades de 116,3 kDa cada una)
Definición de unidad
One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.
Forma física
Suspension in 50% glycerol, 5 mM Tris buffer salts, 5 mM magnesium chloride, 0.5 mM DTT, 0.5 mM mercaptoethanol. pH 7.4
Otras notas
Loss of enzymatic activity can occur if this enzyme is frozen. This enzyme is provided in a 50% glycerol solution and will not freeze if stored at -20° Celsius. It is not recommended that this enzyme preparation be stored in an ultracold freezer (-60 to -100° Celsius).
Inhibidor
Referencia del producto
Descripción
Precios
sustrato
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Certificados de análisis (COA)
Lot/Batch Number
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H Morreau et al.
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We have isolated two cDNAs encoding human lysosomal beta-galactosidase, the enzyme deficient in GM1-gangliosidosis and Morquio B syndrome, and a beta-galactosidase-related protein. In total RNA from normal fibroblasts a major mRNA of about 2.5 kilobases (kb) is recognized by cDNA
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Could you please advise if the density of the aqueous suspension of beta galactosidase from Escherichia coli (G4155) can be assumed as 1 uL of solution is equal to 1 mg, given that the SDS and provided enzyme assay do not contain this information?
1 respuesta-
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We recently purchased this enzyme. Information given regarding enzyme volume is only 1000 units. I want to know what would be units per uL. How much is the liquid volume in the given product to calculate the enzyme concentration? Provide proper informatio
1 respuesta-
Units/ml can be calculated using the numbers reported in batch specific CofA. Below is an example.
CofA of G4155, lot 0000303635
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/G4155-3KU0000303635.pdf
1.5 mgP/ml X 766 units/mg Protein = 1149 units/ml¿Le ha resultado útil?
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