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F3040

Monoclonal ANTI-FLAG^® M1 antibody

Name: Monoclonal ANTI-FLAG<SUP>®</SUP> M1 antibody
Brand: SIGMA
Price: 527 EUR

clone M1, purified immunoglobulin, buffered aqueous solution

Sinónimos:

Anti-ddddk, Anti-dykddddk

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Talla/SKU	Disponibilidad	Precio
0.2 mg	Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad	527,00 €
1 mg	Disponible para enviar HOYdesdeLyon	1010,00 €
5 mg	Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad	1800,00 €

Acerca de este artículo

UNSPSC Code:

12352203

NACRES:

NA.32

MDL number:

MFCD00212941

Conjugate:

unconjugated

Clone:

M1, monoclonal

Application:

Citations:

139

527,00 €

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Propiedades

biological source

(Bioreactor)

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M1, monoclonal

form

buffered aqueous solution

purified by

using Protein A

species reactivity

all

concentration

2-5 mg/mL

technique(s)

western blot: 10 μg/mL

immunogen sequence

DYKDDDDK

shipped in

dry ice

storage temp.

−20°C

Descripción

General description

The ANTI-FLAG M1 IgG2b mooclonal antibody binds to proteins with a FLAG marker at the free N-terminus.

Method of purification - Protein A

Immunogen

FLAG; peptide sequence DYKDDDDK

Application

Monoclonal ANTI-FLAG^® M1 antibody is suitable for immunoprecipitation, western blotting, or EIA in yeast, animal and E.coli cells.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.

Chromatin immunoprecipitation (1 paper)

Immunofluorescence (1 paper)

Learn more product details in our FLAG^® applications portal.

Biochem/physiol Actions

Binds to the FLAG epitope when it is located at the free amino-terminus of a fusion protein. Does not bind to Met-FLAG fusion proteins, so will not recognize unprocessed, cytoplasmically expressed proteins. Binding is Ca²⁺-dependent; the complex dissociates in the absence of calcium ions.

Physical form

Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v).

Preparation Note

Dilute antibody to 10ug/mL in .05 M TBS, pH 7.4, containing 1 mM CaCl2.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Este artículo	A9594	F9291	F1804
Sigma-Aldrich F3040 Monoclonal ANTI-FLAG^® M1 antibody	Sigma-Aldrich A9594 ANTI-FLAG^® M2 monoclonal antibody produced in mouse	Sigma-Aldrich F9291 ANTI-FLAG^® M2 monoclonal antibody produced in mouse	Sigma-Aldrich F1804 ANTI-FLAG^® M2 monoclonal antibody produced in mouse
description clone M1, purified immunoglobulin, buffered aqueous solution	description clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)	description clone M2, purified immunoglobulin, buffered aqueous glycerol solution	description 1.0-1.2 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
conjugate unconjugated	conjugate CY3 conjugate	conjugate biotin conjugate	conjugate unconjugated
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form affinity isolated antibody
clone M1, monoclonal	clone M2, monoclonal	clone M2, monoclonal	clone M2, monoclonal
biological source (Bioreactor)	biological source mouse	biological source mouse	biological source mouse
species reactivity all	species reactivity all	species reactivity all	species reactivity all
technique(s) western blot: 10 μg/mL	technique(s) direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone	technique(s) dot blot: suitable (chemiluminescent detection)	technique(s) -

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