E5389

Erythromycin

powder, suitable for cell culture, BioReagent

• Sinónimos: E-Mycin, Erythrocin
• Fórmula empírica (notación de Hill): C₃₇H₆₇NO₁₃
• Número de CAS: 114-07-8
• Peso molecular: 733.93
• Beilstein: 75279
• Número CE: 204-040-1
• Número MDL: MFCD00084654
• Código UNSPSC: 12352207
• ID de la sustancia en PubChem: 24894612
• NACRES: NA.76

Propiedades

• Nombre del producto: Erythromycin, BioReagent, suitable for cell culture
• Línea del producto: BioReagent
• Nivel de calidad: 200
• Formulario: powder
• potencia: ≥850 μg per mg
• técnicas: cell culture | mammalian: suitable
• impurezas: ≤0.1 EU/mg endotoxin
• color: white
• mp: 133 °C
• solubilidad: 2 M HCl: 50 mg/mL (Stock solutions should be stored at 2-8 °C. Stable at 37 °C for 3 days.); ethanol: soluble (Stock solutions should be stored at 2-8 °C. Stable at 37 °C for 3 days.)
• espectro de actividad antibiótica: Gram-negative bacteria; Gram-positive bacteria
• Modo de acción: protein synthesis | interferes
• cadena SMILES: CC[C@H]1OC(=O)[C@H](C)[C@@H](O[C@H]2C[C@@](C)(OC)[C@@H](O)[C@H](C)O2)[C@H](C)[C@@H](O[C@@H]3O[C@H](C)C[C@@H]([C@H]3O)N(C)C)[C@](C)(O)C[C@@H](C)C(=O)[C@H](C)[C@@H](O)[C@]1(C)O
• InChI: 1S/C37H67NO13/c1-14-25-37(10,45)30(41)20(4)27(39)18(2)16-35(8,44)32(51-34-28(40)24(38(11)12)15-19(3)47-34)21(5)29(22(6)33(43)49-25)50-26-17-36(9,46-13)31(42)23(7)48-26/h18-26,28-32,34,40-42,44-45H,14-17H2,1-13H3/t18-,19-,20+,21+,22-,23+,24+,25-,26+,28-,29+,30-,31+,32-,34+,35-,36-,37-/m1/s1
• Clave InChI: ULGZDMOVFRHVEP-RWJQBGPGSA-N
• Información sobre el gen: human ... ABCB1(5243) , CYP3A4(1576) , MLNR(2862) ; mouse ... Abcb1a(18671) , Abcb1b(18669)

Descripción

Descripción general

Chemical structure: macrolide

Erythromycin is an antibiotic produced by growth of certain strains of Streptomyces erythreus. This product is composed largely of erythromycin A with small amounts of erythromycins B and C and is recommended for concentration at 100 mg/L. Concentrations between 50 and 200 mg/L have also proven effective in controlling bacterial growth. Erythromycin has been used as a motilin receptor agonist, to block respiratory glycoconjugate secretion in human airways in vitro, and for selecting plasmid-cured and recombinant lactococcus lactis MG1363 strains.

Aplicación

Erythromycin has been used:

• as a supplement for nutrient broth medium for culturing green fluorescent protein (GFP)- expressing E. coli[1]
• as a model drug to determine small intestinal (SMI) microtissue viability using the MTT assay{254
• as an antibiotic to study the treatment strategies of chronic infections[2]

Acciones bioquímicas o fisiológicas

Mode of Action: Erythromycin acts by inhibiting elongation at the transpeptidation step, specifically aminoacyl translocation from the A-site to P-site by binding to the 50s subunit of the bacterial 70s rRNA complex.

Antimicrobial Spectrum: This product acts against both gram-negative and gram-positive bacteria.

Precaución

This product is stable in solution at 37°C for 3 days. Stock solutions should be stored at 2-8°C.

Nota de preparación

This product is soluble in water at 2 mg/mL, with a 0.067% solution in water yielding a pH of 8.0-10.5. It is also soluble in ethanol at 50 mg/mL, yielding a clear, colorless to faint yellow solution. It is freely soluble in alcohol, acetone, chloroform, acetonitrile and ethyl acetate but forms salts with acids. All solutions should be protected from light.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)