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Merck

D8764

Sigma-Aldrich

Deoxyribonuclease II from bovine spleen

Type V, essentially salt-free, lyophilized powder, ≥1,000 units/mg protein

Sinónimos:

DNase II, Deoxyribonucleate 3′-oligonucleotido-hydrolase

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30000 UNITS
123,00 €
150000 UNITS
355,00 €
300000 UNITS
604,00 €

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30000 UNITS
123,00 €
150000 UNITS
355,00 €
300000 UNITS
604,00 €

About This Item

Número de CAS:
Comisión internacional de enzimas:
Número CE:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54

123,00 €


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origen biológico

bovine spleen

tpo

Type V

Formulario

essentially salt-free, lyophilized powder

actividad específica

≥1,000 units/mg protein

mol peso

38 kDa

concentración

≥50% protein

técnicas

DNA extraction: suitable
tissue culture: suitable

idoneidad

suitable for molecular biology

aplicaciones

cell analysis

actividad extraña

RNase ≤0.1%

temp. de almacenamiento

−20°C

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Aplicación

Deoxyribonuclease II from bovine spleen has been used:
  • as a component of digestion mix to isolate immune cells from colon samples[1]
  • to digest retinal explants for single-cell suspension preparation[2]
  • for isolation of stromal cells from human colon tissue[3]
  • in the dissociation medium during the preparation of embryonic cardiac myocytes from rat heart[4]

Acciones bioquímicas o fisiológicas

Deoxyribonuclease II, also called as acid DNAse, hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3′-phosphates. in vitro, its optimum pH range is 4.5 - 5.0. It also acts upon p-nitrophenyl-phosphodiesters at pH 5.6 - 5.9. The molecular weight is approximately 38,000 Da.[5]

Definición de unidad

One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25 °C; [Mg2+] = 0.83 mM

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Certificados de análisis (COA)

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K Tempel
Arzneimittel-Forschung, 30(7), 1119-1123 (1980-01-01)
The influence of three mucopolysaccharide-polysulfuric acid-esters (MPS) of different molecular weight on DNAase II-activity as well as on nucleic acid and protein synthesis of lymphocytes of rat spleen has been investigated in vitro. The results are summarized as follows: 1.
STUDIES ON ACID DEOXYRIBONUCLEASE. II. ISOLATION AND CHARACTERIZATION OF SPLEEN-ACID DEOXYRIBONUCLEASE.
G BERNARDI et al.
Biochemistry, 3, 1419-1426 (1964-10-01)
Dopamine D2 receptor stimulation differentially affects voltage-activated calcium channels in rat pituitary melanotropic cells.
Keja J A, et al.
The Journal of Physiology, 450(1), 409-435 (1992)
S Ingebrandt et al.
Biosensors & bioelectronics, 16(7-8), 565-570 (2001-09-07)
An extracellular recording system has been designed for the detection of electrical cell signals using p-channel or n-channel field-effect transistor (FET) arrays with non-metallized gates. Signals from rat heart muscle cell were recorded by these devices and the results described
K Nitta et al.
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, 114(2), 119-128 (1994-02-01)
Deoxyribonucleases from eggs and the liver of Xenopus laevis were partially purified by DEAE-cellulose and heparin-Sepharose affinity column chromatographies. The fractions having egg and liver DNase activities were eluted on high performance liquid chromatography through TSK gel G3000SW at the

Preguntas

  1. What is the best buffer to dissolve the lyophilized powder in? What is the best final concentration to be prepared?

    1 respuesta
    1. According to the Enzymatic Assay Test Procedure, a solution should be prepared immediately before use, containing 500–1000 Kunitz units/ml in cold deionized water. More information can be found in the document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/769/284/d8764enz.pdf

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