This formulation contains 100 mg/L of Magnesium Chloride Heptahydrate and 133 mg/L of Calcium Chloride Dihydrate. Please see the link below to review the full formulation of this and other Dulbecco's buffer products:
https://www.sigmaaldrich.com/product/sigma/d8662#product-documentation
D8662
Disolución salina tamponada con fosfatos de Dulbecco
Dulbecco’s Phosphate Buffered Saline
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Liquid, with MgCl₂ and CaCl₂, sterile-filtered, suitable for cell culture
Sinónimos:
DPBS
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100 ML
12,80 €
500 ML
22,50 €
1 L
41,50 €
12,80 €
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100 ML
12,80 €
500 ML
22,50 €
1 L
41,50 €
About This Item
12,80 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Solicitar un pedido a granel
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Nombre del producto
Disolución salina tamponada con fosfatos de Dulbecco, With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture
Nivel de calidad
esterilidad
sterile-filtered
Formulario
liquid
técnicas
cell culture | mammalian: suitable
impurezas
endotoxin, tested
componentes
sodium pyruvate: no
glucose: no (Dextro)
Condiciones de envío
ambient
temp. de almacenamiento
2-8°C
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Descripción general
Dulbecco′s Phosphate Buffered Saline (D-PBS) is a balanced salt solution developed for use in tissue culture.
Aplicación
D-PBS is a general use isotonic saline solution used to wash cells during preparation and serial transfer.
Dulbecco′s Phosphate Buffered Saline has been used:
- as a component of the reaction mixture for the fluoro-labeling reaction of pro-nerve growth factor (proNGF)-YBBR
- to wash and equilibrate the cells post transepithelial electrical resistance (TEER) measurement of Caco-2BBe cells
- to wash cells for protein isolation
Dulbecco′s Phosphate Buffered Saline has been used:
- as a component of the reaction mixture for the fluoro-labeling reaction of pro-nerve growth factor (proNGF)-YBBR[1]
- to wash and equilibrate the cells post transepithelial electrical resistance (TEER) measurement of Caco-2BBe cells[2]
- to wash cells for protein isolation[2]
Acciones bioquímicas o fisiológicas
Dulbecco′s Phosphate Buffered Saline (D-PBS) exhibits four chief functions:
- provides cells with water and certain bulk inorganic ions essential for normal cell metabolism
- serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance
- when combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism
- provides a buffering system to maintain the medium within the physiological pH range
Producto relacionado
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
nwg
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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What is the concentration of MgCl2 and CaCl2?
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¿Disponen de algún estudio de estabilidad de este producto a temperatura Ambiente? (15 - 30 ºC)
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Any stability study information is considered proprietary and may not be shared. However, this sterile DPBS solution is quite stable. The product is assigned an expiration date of approximately 2 years from the date of quality release. The item ships at ambient temperature and is stable for up to two weeks to allow for transit time. For long term storage, refrigeration at 2-8°C is recommended.
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