See the link below to review the formulations of the DPBS products. These concentrations are reported in grams per liter:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/269/385/d5652for.pdf
D5652
Disolución salina tamponada con fosfatos de Dulbecco
Modified, without calcium chloride and magnesium chloride, powder, suitable for cell culture
Sinónimos:
DPBS
Iniciar sesión para ver los precios por organización y contrato.
Seleccione un Tamaño
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Acerca de este artículo
Saltar a
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarlepH
7.2-7.8
form
powder
technique(s)
cell culture | mammalian: suitable
solubility
water: 9.6 g/L, clear
storage temp.
2-8°C
Quality Level
¿Está buscando productos similares? Visita Guía de comparación de productos
Categorías relacionadas
1 of 4
Este artículo | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| Quality Level 500 | Quality Level 400 | Quality Level 400 | Quality Level 200 |
| form powder | form liquid | form liquid | form liquid |
| solubility water: 9.6 g/L, clear | solubility - | solubility - | solubility - |
| storage temp. 2-8°C | storage temp. - | storage temp. 2-8°C | storage temp. 2-8°C |
| pH 7.2-7.8 | pH - | pH - | pH - |
General description
Dulbecco′s Phosphate Buffered Saline is a balanced salt solution (BSS) widely used in tissue culture.
Application
D-PBS is used to wash cells during preparation and serial transfer.
Dulbecco′s Phosphate Buffered Saline has been used:
- as one of the pseudo-physiological solutions to study the in vitro degradation behavior of Fe–20Mn–1.2C alloy[1]
- to dissolve Angelica gigas powder for microencapsulation[2]
- to wash cells as part of cell culture treatments, wound-healing assay, transwell assay and apoptosis assay[3]
- to wash amniotic membrane for cellular analyses[4]
Biochem/physiol Actions
Dulbecco′s Phosphate Buffered Saline serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance. It hydrates cells and acts as a source of bulk inorganic ions essential for normal cell metabolism. This buffered saline acts as the principal energy source for cell metabolism when combined with a carbohydrate, such as glucose. It provides a buffering system to maintain the medium within the physiological pH range.
Preparation Note
Formulated to contain 9.6 grams of powder per liter of medium.
Other Notes
This D-PBS is formulated without Ca or Mg. Formulations with Ca and Mg added are also available
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Ana C Bohórquez et al.
Journal of colloid and interface science, 506, 393-402 (2017-07-28)
The rotational diffusion of polyethylene glycol coated magnetic nanoparticles in serum albumin solutions was investigated in a range spanning 0mgmL
Zhaoxia Zhang et al.
Molecular vision, 24, 434-442 (2018-07-24)
Retinal G protein-coupled receptor (RGR) mRNA is transcribed in the outer nuclear layer of human retinas; however, it is not known whether the RGR gene is expressed in the rod or cone photoreceptors. In this study, we investigate broader expression
Nunki Hassan et al.
STAR protocols, 1(3), 100156-100156 (2020-12-31)
Patient-derived xenografts (PDXs) are the most valuable tool for preclinical drug testing because they retain the genetic diversity and phenotypic heterogeneity of the original tumor. Acute myeloid leukemia (AML) remains difficult to engraft in immunodeficient mice. This is particularly true
Barbora Bílková et al.
Veterinary immunology and immunopathology, 188, 71-77 (2017-06-16)
While haematological variation is well known in birds, variation in avian breeds (distinct morphotypes of the same species) remains unexplored. Poultry breeds, in particular, may show interesting evolutionary patterns and economically-relevant physiological differences. We performed a comparative examination of blood
Y Naka et al.
Materials today. Bio, 6, 100054-100054 (2020-06-02)
The in vitro fabrication of wholly vascularized millimeter-sized engineered tissues is still a key challenge in the tissue engineering field. Recently we reported a unique approach 'sedimentary culture' using a collagen microfiber (CMF) to fabricate large-scale engineered tissues. The millimeter-sized
Contenido relacionado
Instructions
-
What is the formulation for this buffer? Molarity of Na2HPO4, KH2PO4, NaCl, KCl, etc?
1 respuesta-
¿Le ha resultado útil?
-
-
What is the pH of Product D5652, Dulbecco's Phosphate Buffered Saline?
1 respuesta-
The specification for the pH of D5652, Dulbecco's Phosphate Buffered Saline (when dissolved in water) is 7.2 to 7.8.
¿Le ha resultado útil?
-
-
Is Product D5652, Dulbecco's Phosphate Buffered Saline, a 1X solution once it has been diluted?
1 respuesta-
Product No. D5652, DPBS is a 1X solution after reconstitution at 9.6 g/L.
¿Le ha resultado útil?
-
-
What is the Department of Transportation shipping information for this product?
1 respuesta-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
¿Le ha resultado útil?
-
-
Is Product D5652, Dulbecco's Phosphate Buffered Saline, offered as a powder or a liquid?
1 respuesta-
Product No. D5652, Dulbecco's Phosphate Buffered Saline, is a powder.
¿Le ha resultado útil?
-
-
Can Product D5652, Dulbecco's Phosphate Buffered Saline, be autoclaved?
1 respuesta-
Product No. D5652, Dulbecco's Phosphate Buffered Saline should be autoclaved or sterile-filtered before use. It can be autoclaved at 121°C, 15 psi for 20 minutes.
¿Le ha resultado útil?
-
-
What is the sodium chloride concentration in Product D5652, Dulbecco's Phosphate Buffered Saline?
1 respuesta-
This product contains 8.0 g/L sodium chloride.
¿Le ha resultado útil?
-
Filtros activos
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico