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D2532

Sigma-Aldrich

Denhardt′s Solution 50x

for Northern and Southern blotting, solution

Sinónimos:

blocking reagent, blocking solution, denhardts, hybridization buffer, hybridization solution, northern blocking solution, pre-hybridization, southern blocking solution

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5 ML
234,00 €
5 X 5 ML
896,00 €

234,00 €

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5 ML
234,00 €
5 X 5 ML
896,00 €

About This Item

Código UNSPSC:
12161700
NACRES:
NA.25

234,00 €

Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad

Nivel de calidad

300

Formulario

solution

técnicas

Southern blotting: suitable

actividad extraña

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

temp. de almacenamiento

−20°C

Categorías relacionadas

Descripción general

Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This 50x concentrate can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.

Componentes

Denhardt′s Solution is 1% Bovine Serum Albumin (BSA), 1 % Ficoll, and 1 % Polyvinylpyrrolidone (PVP) in water.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000375543
SLCN0620
0000320236
0000317838
0000308209

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Preguntas

  1. Can you share the CAS # for the components (Ficoll and PVP) that are used to make the 50X Denhart's Solution?

    1 respuesta

    1. The CAS number for the Ficoll ingredient is 26873-85-8. The CAS number for the PVP ingredient is 9003-39-8 2.

      Below is the link to the Product Data Sheet for more information: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/172/670/d2532dat.pdf

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