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D0428

Sigma-Aldrich

1 kb DNA Ladder

for DNA electrophoresis

Sinónimos:

1kb gel ladder, 1kb gel marker, 1kb ladder for gel electrophoresis, DNA gel marker, agarose gel electrophoresis ladder, agarose gel electrophoresis marker

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About This Item

Código UNSPSC:
41105335
NACRES:
NA.25

Nivel de calidad

100

formulario

liquid

idoneidad

suitable for electrophoresis (DNA)

temp. de almacenamiento

−20°C

Categorías relacionadas

Descripción general

Sigma′s 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb, and 2 kb repeats from 6 to 10 kb. In nucleic acid gel electrophoresis, five μl of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting. All DNA markers can be stained with ethidium bromide, SYBR® Green, and Nancy-520.

Aplicación

1 kb DNA Ladder has been used as a molecular marker to determine the molecular weight and size of double stranded DNA during gel electrophoresis.

Componentes

Sigma′s 1kb Ladder is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Otras notas

For optimal resolution, the recommended agarose gel concentration is 0.75%.

Información legal

SYBR is a registered trademark of Life Technologies

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Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

10 - Combustible liquids

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Gel Electrophoresis Markers

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

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