CWA-1056

Anti-Human CD11c (3.9) ColorWheel^® Dye-Ready mAb

for use with ColorWheel^® Dyes (Required, sold separately)

• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 3.9, monoclonal
• Línea del producto: ColorWheel^®
• formulario: lyophilized
• mol peso: calculated mol wt 127.38 kDa
• reactividad de especies: human
• reactividad de especies (predicha por homología): nonhuman primates
• envase: antibody small pack of 25 μL
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• técnicas: flow cytometry: suitable
• isotipo: IgG1κ
• secuencia del epítopo: Unknown
• Nº de acceso Protein ID: NP_001273304.1
• Nº de acceso UniProt: P20702
• compatibilidad: for use with ColorWheel^® Dyes (Required, sold separately)
• categoría alternativa más sostenible: Aligned,
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... ITGAX;CD11C(3687)

Descripción

Descripción general

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Especificidad

Clone 3.9 is a mouse monoclonal antibody that detects Integrin alpha-X (CD11c). It targets an epitope within the I-domain from the N-terminal half.

Inmunógeno

Human monocytes and synovial cells.

Aplicación

Quality Control Testing:

Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).

Staining of one million human peripheral blood mononuclear cells (PBMC) was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1056, Anti-Human CD11c (3.9) ColorWheel^® Dye-Ready mAb and Cat. No. CWD-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or the equivalent amount of PE-conjugated Mouse IgG1 isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Compatibilidad

Requires ColorWheel^® Dye (Sold Separately)

Descripción de destino

Integrin alpha-X (UniProt: P20702; also known as CD11 antigen-like family member C; Leu M5; Leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor p150-95; CD11c) is encoded by the ITGAX (also known as CD11C) gene (Gene ID: 3687) in human. CD11c is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to generate the mature form that contains a long extracellular domain (aa 20-1107), a transmembrane domain (aa 1108-1128), and a cytoplasmic domain (aa 1129-1163). CD11c is predominantly expressed in macrophages, monocytes, and granulocytes and along with integrin β2 it serves as a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. The αXβ2 heterodimer binds many ligands, including collagen type 1, iC3b, fibrinogen, ICAM-1, and lipopolysaccharide. It is reported to mediate cell-cell interaction during inflammatory responses and is especially important in monocyte adhesion and chemotaxis. The I domains of CD11 are responsible for the binding of ligands and have a unique structure with 6-7 α-helixes and 6β sheets with interconnecting loops. In CD11c the loops on the I domain are involved in fibrinogen binding and loops α3-α4 and βD-α5 are reported to be more important for recognition of fibrinogen. (Ref.: Vorup-Jensen, T., et al. (2003). Proc. Natl. Acad. Sci. USA. 100(4); 1873-1878; Choi, J., and Nham, S-U. (2002). Biochem. Biophys. Res. Commun. 292(3); 756-760).

Forma física

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

Reconstitución

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

Información legal

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable