CTLL-2

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93042610, mouse blood, Lymphoblast

• Sinónimos: CTLL-2 cell line
• Código UNSPSC: 41106514

Propiedades

• origen biológico: mouse blood
• envase: tube of 5 μg 93042610-DNA-5UG; pkg of vial of cells 93042610-1VL
• modo de crecimiento: Suspension
• cariotipo: Not specified
• morfología: Lymphoblast
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse C57b1/6 T cell

Descripción línea celular

CTLL is a subclone of T cells derived from a C57bl/6 mouse. The cells require IL-2 for growth and are used to assay for its presence in conditioned media.

Aplicación

CTLL-2 has been used in CTLL-2 cell proliferation assay to determine the presence of T-cell cytokines.[1][2]

Medio de cultivo

RPMI 1640 + 2mM Glutamine + 10-20 IU/ml Human Recombinant IL-2 + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Maintain cultures between 3-9 x 10⁵ cells/ml; 5% CO₂; 37°C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 4-5 x 10⁵ cells/ml.

Otras notas

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