CHO Cell Line from Chinese hamster ovary

85050302, hamster ovary, Epithelial

• Sinónimos: CHO Cells, CHO-ori Cells, Chinese Hamster Ovary Cells, Chinese hamster ovary cell line
• Código UNSPSC: 41106514
• origen biológico: hamster ovary
• modo de crecimiento: Adherent
• cariotipo: Hypodiploid, modal no. 20
• morfología: Epithelial
• productos: Not specified
• receptores: Not specified

Propiedades

• Nombre del producto: CHO Cell Line from Chinese hamster ovary, 85050302, from hamster ovary
• origen biológico: hamster ovary
• disponibilidad: not available in Japan
• modo de crecimiento: Adherent
• cariotipo: Hypodiploid, modal no. 20
• morfología: Epithelial
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Hamster Chinese ovary

Descripción línea celular

A subclone of the parental CHO cell line originated by Puck in 1957. The cells have an absolute requirement for L-proline.

Aplicación

CHO Cell Line from Chinese hamster ovary has been used:

• to generate CHO cells expressing LY6/PLAUR domain containing 3 (LYPD3)
• to carry out the vaginolysin (VLY) cytolytic activity assay
• as host cells to produce glucagon-like peptide 1 receptor (GLP1R)

Nutritional and gene expression studies

Medio de cultivo

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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