UM-RC-3

8090512, human kidney (renal gland), Polygonal

• Código UNSPSC: 41106514

Propiedades

• product name: UM-RC-3, 08090512
• origen biológico: human kidney (renal gland)
• modo de crecimiento: Adherent
• cariotipo: Median number of chromosomes is 55
• morfología: Polygonal
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human renal carcinoma

Descripción línea celular

UM-RC-3 is a continuous cell line established from a primary renal adenocarcinoma. It exhibits a loss of contact inhibition and produces tumours in the nude mouse model. The patient had metastatic disease at the time of their nephrectomy.

Aplicación

Study of tumour associated antigens.

Perfil del ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 9,12
D16S539: 9
D5S818: 9,12
D7S820: 9
THO1: 9.3
TPOX: 8
vWA: 16,18

Medio de cultivo

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 1-2x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C. Doubling time approximately 29 hours.

Otras notas

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Cláusula de descargo de responsabilidad

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.