RCP

31626, rat brain, Endothelial

• Código UNSPSC: 41106514

Propiedades

• product name: RCP, 00031626
• origen biológico: rat brain
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Endothelial
• productos: Not specified
• receptores: Vasopressin V1a, Angiotensin II, Endothelin 1, Bradykinin, Serotonin
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Rat Choroid Plexus

Descripción línea celular

The choroid plexus of adult Wistar Rats were dissected from the fourth ventricle to give a primary culture. This primary culture was then immortalized by transfection with the TSori minus adenovirus (a replication deficient adenovirus containing SV40 large T antigen). Sub-cloning of the immortalised cells was performed by selecting for clones which exhibited calcium ion release in response to vasopressin, angiotensin, bradykinin, serotonin or endothelin stimulation. The sub-clone which exhibited the largest response to all five hormones was selected and sub-cultured on to become RCP.

Aplicación

Toxicology, cellular signalling and pharmacology studies

Medio de cultivo

DMEM (D6546) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442) + 0.1% Epidermal Growth Factor(EGF) (E9644), 1% Insulin–Transferrin–Selenium(ITS) (I3146)

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C

Otras notas

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