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Merck

CASP3F

Caspase 3 Assay Kit, Fluorimetric

Sinónimos:

Caspase-3 Activity Fluorometric Kit

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UNSPSC Code:
12161503
EC Number:
247-454-8
NACRES:
NA.84

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usage

sufficient for 1,000 multiwell tests, sufficient for 100 standard tests (2 ml)

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

Gene Information

human ... CASP3(836)

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Este artículo
CASP3CAPT131APT165
Caspase 3 Assay Kit, Fluorimetric

Sigma-Aldrich

CASP3F

Caspase 3 Assay Kit, Fluorimetric

Caspase 3 Assay Kit, Colorimetric

Sigma-Aldrich

CASP3C

Caspase 3 Assay Kit, Colorimetric

Caspase 3 Colorimetric Activity Assay Kit, DEVD Caspase-3 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence DEVD.

Sigma-Aldrich

APT131

Caspase 3 Colorimetric Activity Assay Kit, DEVD

Caspase 3 Colorimetric Activity Assay Kit, DEVD Caspase-3 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence DEVD.

Sigma-Aldrich

APT165

Caspase 3 Colorimetric Activity Assay Kit, DEVD

Gene Information

human ... CASP3(836)

Gene Information

human ... CASP3(836)

Gene Information

human ... CASP3(836)

Gene Information

human ... CASP3(836)

Quality Level

200

Quality Level

300

Quality Level

100

Quality Level

100

usage

sufficient for 1,000 multiwell tests, sufficient for 100 standard tests (2 ml)

usage

sufficient for 1,000 multiwell tests (in 96-well multiwell plates), sufficient for 100 standard tests (1 ml)

usage

-

usage

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

storage temp.

-

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

General description

The Caspase 3 Fluorometric Assay Kit is based on the hydrolysis of acetyl Asp-Glu-Val-Asp 7-amido-4-methylcoumarin (Ac-DEVD-AMC) by caspase 3, resulting in the release of the fluorescent 7-amino-4-methylcoumarin (AMC). The excitation and emission wavelengths of AMC are 360 nm and 460 nm respectively. The concentration of the AMC released can be calculated from a calibration curve prepared with AMC standards (AMC standard included with the kit).

Application

The Caspase 3 Fluorimetric Assay Kit is designed for the detection and measurement of caspase 3 activity in cell extracts or purified preparations of caspase 3.

Other Notes

One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD per min at pH 7.4 at 25 °C.

Solo componentes del kit

Referencia del producto
Descripción
  • Water (17-18 megaOhm) 125 mL

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS
  • A1086Ac-DEVD-AMC substrate 2.5 mgSDS
  • A0835Ac-DEVD-CHO inhibitor .5 mgSDS
  • A98917-Amino-4-methylcoumarin standard 1 mgSDS

related product

Referencia del producto
Descripción
Precios

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 3

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
0000412229
0000412229
0000212536
0000212536
0000111687

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Radharaman Ray et al.
Drug and chemical toxicology, 28(1), 105-116 (2005-02-22)
Sulfur mustard [SM; bis-(2-chloroethyl) sulfide], which causes skin blistering or vesication [(1991). Histo- and cytopathology of acute epithelial lesions. In: Papirmeister, B., Feister, A. J., Robinson, S. I., Ford, R. D., eds. Medical Defense Against Mustard Gas: Toxic Mechanisms and
V A Smith et al.
The British journal of ophthalmology, 88(5), 619-625 (2004-04-20)
Doxycycline is a broad spectrum antibiotic that chelates metal ions and is frequently used as part of the treatment of ocular surface diseases. Its therapeutic value has been ascribed to an ability to inhibit matrix metalloproteinase (MMP) activity and both
Sau Wai Hung et al.
PloS one, 8(8), e71196-e71196 (2013-08-14)
We evaluated the potential of an investigational histone methylation reversal agent, 3-deazaneplanocin A (DZNep), in improving the chemosensitivity of pancreatic cancer to nucleoside analogs (i.e., gemcitabine). DZNep brought delayed but selective cytotoxicity to pancreatic cancer cells without affecting normal human
Irshad Ali et al.
The Annals of thoracic surgery, 93(1), 282-288 (2011-11-26)
We previously reported that the cytochrome P450 product 20-hydroxyeicosatetraenoic acid has prosurvival effects in pulmonary artery endothelial cells and ex vivo pulmonary arteries. We tested the potential of a 20-hydroxyeicosatetraenoic acid analog N-[20-hydroxyeicosa-5(Z),14(Z)-dienoyl]glycine (20-5,14-HEDGE) to protect against lung ischemic reperfusion
N M Martins et al.
Journal of applied toxicology : JAT, 28(3), 337-344 (2007-07-03)
Cisplatin is a potent and widely used chemotherapeutic agent. Nephrotoxicity induced by this drug has been well documented. However, very little information is available on cisplatin-induced hepatotoxicity and its underlying mechanism remains unclear. High doses of cisplatin have been known
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