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CASP3C

Caspase 3 Assay Kit, Colorimetric

Sinónimos:

Colorimetric Caspase-3 Activity Assay Kit

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A ustedes/SKUDisponibilidadPrecio
1 kit
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968,00 €

Acerca de este artículo

NACRES:
NA.84
UNSPSC Code:
12161503

968,00 €


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Quality Segment

usage

sufficient for 1,000 multiwell tests (in 96-well multiwell plates), sufficient for 100 standard tests (1 ml)

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CASP3(836)

General description

Caspase 3 Colorimetric Assay Kit provides the reagents needed for a quick and efficient detection of caspase 3 activity in cell lysates and in purified preparations of caspase 3.

The Caspase 3 Colorimetric Assay Kit is based on the hydrolysis of acetyl-Asp-Glu-Val-Asp p-nitroanilide (Ac-DEVD-pNA) by caspase 3, resulting in the release of the p-nitroaniline (pNA) moiety. p-Nitroaniline is detected at 405 nm (εmM=10.5). The concentration of the pNA released from the substrate is calculated from either the absorbance values at 405 nm or from a calibration curve prepared with pNA standards (pNA standard included with the kit).

Application

The assay can be performed (a) in 1 mL volume and measured using a spectrophotometer, or (b) in 100 µL volume in a 96-well plate using an ELISA reader.

Other Notes

One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD-pNA per min at pH 7.4 at 25 °C.
Use 17 megohm water only

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CASP3FAPT131APT165
Gene Information

human ... CASP3(836)

Gene Information

human ... CASP3(836)

Gene Information

human ... CASP3(836)

Gene Information

human ... CASP3(836)

Quality Level

300

Quality Level

200

Quality Level

100

Quality Level

100

usage

sufficient for 1,000 multiwell tests (in 96-well multiwell plates), sufficient for 100 standard tests (1 ml)

usage

sufficient for 1,000 multiwell tests, sufficient for 100 standard tests (2 ml)

usage

-

usage

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

storage temp.

-

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice


Solo componentes del kit

Referencia del producto
Descripción

  • Water (17-18 megaOhm) 125 mL

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS & Precios

  • Ac-DEVD-CHO inhibitor .5 mg
    SDS

  • Ac-DEVD-pNA substrate 15 mg
    SDS

  • p-Nitroaniline standard 1 mg
    SDS

pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 3 - STOT RE 2

Clase de almacenamiento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

415.4 °F - closed cup

flash_point_c

213 °C - closed cup



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Preguntas

1–3 de 3 Preguntas  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 respuesta
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      ¿Le ha resultado útil?

  2. Hola, Me gustaría saber si este kit sirve para detectar activación de caspasa 3 en células adherentes de insulinoma de ratón. En general en células adherentes de ratón. gracias

    1 respuesta
    1. No data is available regarding the use of this kit with adherent cells; the kit was only tested using Jurkat cells in suspension. For possible usage with adherent cells, it is suggested to consult the literature. Since detachment of adhered cells might induce spontaneous apoptosis, determining the correct conditions for experimental cell induction and detachment before the assay is necessary.

      ¿Le ha resultado útil?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 respuesta
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      ¿Le ha resultado útil?

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