C9409
Creatinine Deiminase microbial
lyophilized powder, ≥25 units/mg protein
Sinónimos:
Creatinine Deaminase
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250 UNITS
877,00 €
877,00 €
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About This Item
Número de CAS:
Número CE:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54
877,00 €
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Formulario
lyophilized powder
actividad específica
≥25 units/mg protein
mol peso
~260 kDa
composición
Protein, ≥15% biuret
temp. de almacenamiento
−20°C
Aplicación
Creatinine Deiminase microbial has been used:
- to immobilize aminosilylated glass beads based biosensor for ammonia/ammonium and creatinine detection in urine[1]
- in creating creatinine-sensing membrane for biophysical studies[2]
- to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples[3]
Creatinine deiminase has been used in a study to assess the application of a creatinine-sensitive biosensor for hemodialysis control. Creatinine deiminase has also been used in a study to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples.
Acciones bioquímicas o fisiológicas
Creatinine deiminase catalyzes the hydrolysis of creatinine to methylhydantoine and ammonia.[1]
Propiedades físicas
Isoelectric point : 4.4
Michaelis constant : 3.5 x 10‾3M (Creatinine)
Structure : 6 subunits per mol of enzyme
Inhibitors : Ag+,Hg++, o-phenanthroline,monoiodoacetate
Optimum pH : 8.5 – 9.5
Optimum temperature : 65 – 75°C
pH Stability : pH 7.0 – 11.0 (30°C, 20hr)
Thermal stability : Below 65°C (pH 7.5, 1hr)
Michaelis constant : 3.5 x 10‾3M (Creatinine)
Structure : 6 subunits per mol of enzyme
Inhibitors : Ag+,Hg++, o-phenanthroline,monoiodoacetate
Optimum pH : 8.5 – 9.5
Optimum temperature : 65 – 75°C
pH Stability : pH 7.0 – 11.0 (30°C, 20hr)
Thermal stability : Below 65°C (pH 7.5, 1hr)
Definición de unidad
One unit will hydrolyze 1.0 μmole of creatinine to N-methylhydantoin and NH3 per min at pH 7.5 at 37 °C in a coupled system with L-glutamic dehydrogenase.
Forma física
Lyophilized powder containing mannitol as stabilizer
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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C M Huang et al.
Clinical chemistry, 34(1), 59-62 (1988-01-01)
We developed an enzymatic method for determination of 5-fluorocytosine in serum, using creatine iminohydrolase (EC 3.5.4.21), the Cobas-Bio analyzer, and an extant ammonia method. Analytical recovery (y) of drug added to serum (x) was good, with y = 0.97x-0.7, Sy.x
Hybrid biosensors for clinical analysis and fermentation control.
I Karube
Methods in enzymology, 137, 131-138 (1988-01-01)
Anne K Bendt et al.
Archives of microbiology, 181(6), 443-450 (2004-05-19)
In order to utilize different nitrogen sources and to survive situations of nitrogen limitation, microorganisms have developed several mechanisms to adapt their metabolism to changes in the nitrogen supply. In this communication, the use of creatinine as an alternative nitrogen
Z K He et al.
Analytical biochemistry, 283(2), 166-174 (2000-07-25)
A precise and sensitive working microflow titration procedure was developed to determine creatinine and ammonia in urine samples. This procedure is based on enzymatic conversion of creatinine, gas diffusional membrane separation of the released ammonia into an acid acceptor stream
R G Washburn et al.
The Journal of antimicrobial chemotherapy, 17(5), 673-677 (1986-05-01)
Creatinine iminohydrolase (EC 3.5.4.21) quantitatively releases ammonia from flucytosine (5FC) as well as from creatinine. Using 39 sera from eight patients receiving the combination of amphotericin B with 5FC, we demonstrated that this rapid enzymatic reaction provides a valid measure
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