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C2409

Creatinase from microorganisms

Name: Creatinase from microorganisms
Brand: SIGMA

lyophilized powder, ≥4 units/mg solid

Sinónimos:

Creatine Amidinohydrolase

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Número CAS:

37340-58-2

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

253-471-1

MDL number:

MFCD00130875

Número CE:

3.5.3.3 (BRENDA, IUBMB)

Specific activity:

≥4 units/mg solid

Biological source:

bacterial (Actinobacillus spp.)

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Propiedades

biological source

bacterial (Actinobacillus spp.)

Quality Segment

200

form

lyophilized powder

specific activity

≥4 units/mg solid

mol wt

~100 kDa

storage temp.

−20°C

Descripción

General description

Creatinase is a homodimer that catalyzes hydrolysis of creatine. It consists of two monomer subunits and two defined domains; N and C terminal domains. The C-terminal fold has both the α helices and anti-parallel β sheet within two structurally similar domains.In between these two domains, a sulfhydryl group acts as active site, and the activity is metal-independent.

Isoelectric point: 4.6 ± 0.1
Michaelis constant: 1.9 x 10‾²M (Creatine)
Structure: 2 subunits per mole of enzyme
Inhibitors: Cu⁺⁺, Hg⁺⁺, Ag⁺
Optimum pH: 8.0
Optimum temp: 40°C
pH Stability: pH 5.5 − 9.0 (25°C, 16hr)
Thermal stability: Below 50°C (pH 7.5, 30 min)

Application

Creatinase mixed with sarcosine oxidase may be used to determine the level of creatine in different pH, temperature, enzyme ratio, and buffer concentration.It may also be used to determine the plasma creatinine level by using a centrifugal analyser.

Biochem/physiol Actions

Creatinase accelerates the conversion reaction of creatine and water molecule to sarcosine and urea. It always acts in homodimer state and is induced by choline chloride.

Physical form

Lyophilized powder containing sugars and EDTA as stabilizers

Other Notes

One unit will hydrolyze 1.0 μmole of creatine to urea and sarcosine per min at pH 7.5 at 37 °C.

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Este artículo	C3172	G8880	2384
Sigma-Aldrich C2409 Creatinase from microorganisms	Sigma-Aldrich C3172 Creatininase from microorganisms	Sigma-Aldrich G8880 Glutaminase from Escherichia coli	Sigma-Aldrich 2384 Creatine Phosphokinase, Rabbit Skeletal Muscle
description lyophilized powder, ≥4 units/mg solid	description lyophilized powder, 100-300 units/mg protein	description Grade V, lyophilized powder, ≥50 units/mg protein	description -
biological source bacterial (Actinobacillus spp.)	biological source -	biological source Escherichia coli	biological source rabbit skeletal muscle
specific activity ≥4 units/mg solid	specific activity 100-300 units/mg protein	specific activity ≥50 units/mg protein	specific activity ≥300 U/mg, 25 °C, 750 U/mg, 37 °C
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized
mol wt ~100 kDa	mol wt ~175 kDa	mol wt 110 kDa	mol wt -
storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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