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C4129

Sigma-Aldrich

α-Chymotrypsin from bovine pancreas

Type II, lyophilized powder, ≥40 units/mg protein

Sinónimos:

α-chymotrypsin A and B, alpha-chymotrypsin

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About This Item

Número de CAS:
9004-07-3
Comisión internacional de enzimas:
3.4.21.1 (BRENDA, IUBMB)
Número CE:
232-671-2
Número MDL:
MFCD00130481
Código UNSPSC:
12352204
eCl@ss:
42010112
NACRES:
NA.54

tpo

Type II

formulario

lyophilized powder

actividad específica

≥40 units/mg protein

mol peso

25 kDa

composición

protein, ≥85%

color

white to off-white

Nº de acceso UniProt

P00767

aplicaciones

diagnostic assay manufacturing

temp. de almacenamiento

−20°C

Información sobre el gen

cow ... CTRB1(618826)

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Categorías relacionadas

Aplicación

The enzyme from Sigma has been used to study the structure-function relationship in glycosylated α-chymotrypsin using immobilized metal-ion affinity chromatography (IMAC) and immobilized metal-ion affinity capillary electrophoresis (IMACE).

Acciones bioquímicas o fisiológicas

α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. The pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10 mM Cu2+ and Hg2+.
A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.

Definición de unidad

One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

Nota de preparación

Produced from 3× crystallized chymotrypsinogen

Nota de análisis

Protein determined by E1%/280

Otras notas

View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer

Aplicación

Referencia del producto
Descripción
Precios

inhibidor

Referencia del producto
Descripción
Precios

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6-Aminocaproic acid, BioUltra, ≥99%

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Diisopropylfluorophosphate

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Trypsin-chymotrypsin inhibitor from Glycine max (soybean), lyophilized powder

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Aprotinina from bovine lung, lyophilized powder, 3-8 TIU/mg solid

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Fluoruro de fenilmetansulfonilo, ≥98.5% (GC)

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Bromoenol lactone, ≥98% (TLC)

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α1-Antitrypsin from human plasma, salt-free, lyophilized powder

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α1-Antitrypsin from human plasma, salt-free, lyophilized powder

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α1-Antichymotrypsin from human plasma, ≥92.5% (SDS-PAGE), lyophilized powder

T4376

N-p-Tosyl-L-phenylalanine chloromethyl ketone, ≥97% (TLC), powder

C7268

Chymostatin, microbial

sustrato

Referencia del producto
Descripción
Precios

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Ines Atuh Ngoh et al.
Experimental parasitology, 218, 107969-107969 (2020-08-29)
Invasion of human red blood cells (RBCs) by Plasmodium parasites is a crucial yet poorly characterised phenotype. Two-color flow cytometry (2cFCM) promises to be a very sensitive and high throughput method for phenotyping parasite invasion. However, current protocols require high
Antonio Jesús Vizcaíno et al.
Marine drugs, 18(6) (2020-06-24)
This piece of research evaluates the presence of protease inhibitors in the macroalga Ulva ohnoi and provides an initial overview of their mode of action. The ability of Ulva protease inhibitors to inhibit digestive proteases of three marine fish species
Shannon Gwala et al.
Food & function, 11(3), 2509-2521 (2020-03-07)
Postharvest storage of legumes is a strategy to ensure food security. However, the hard-to-cook phenomenon, partly explained by pectin-cation-phytate theory, may develop during storage. Based on this theory, minerals could be redistributed within the matrix, affecting the concentration of free/bound
K Y Jiang et al.
Biochimica et biophysica acta, 1433(1-2), 198-209 (1999-08-14)
Chemical glycosylation of bovine alpha-chymotrypsin, by a glucosamine adduct on the carboxyl group, results in the modification of its catalytic activity. The structural alterations of alpha-chymotrypsin resulting from its glycosylation are studied by immobilized metal-ion affinity chromatography (IMAC) and immobilized
Robert Winkler
Rapid communications in mass spectrometry : RCM, 24(3), 285-294 (2010-01-06)
Electrospray ionization (ESI) ion trap mass spectrometers with relatively low resolution are frequently used for the analysis of natural products and peptides. Although ESI spectra of multiply charged protein molecules also can be measured on this type of devices, only
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Artículos

Chymotrypsin

Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.

Protocolos

Procedure for Enzymatic Assay of α-Chymotrypsin (EC 3.4.21.1)

Follow our procedure for the determination of a chymotrypsin activity. This enzymatic assay of alpha chymotrypsin guides you through the entire process and necessary calculations.

Chromatograms

application for HPLC

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