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C3260

Sigma-Aldrich

Citrate Synthase from porcine heart

ammonium sulfate suspension, ≥100 units/mg protein

Sinónimos:

Citrate condensing enzyme, Citrate oxaloacetate lyase (CoA-acetylating)

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About This Item

Número de CAS:
9027-96-7
Comisión internacional de enzimas:
4.1.3.7 (BRENDA, IUBMB)
Número MDL:
MFCD00130808
Código UNSPSC:
12352204
NACRES:
NA.54

origen biológico

Porcine heart

Nivel de calidad

200

Formulario

ammonium sulfate suspension

actividad específica

≥100 units/mg protein

mol peso

98 kDa ( 49 kDa monomer)

solubilidad

H2O: soluble 1.0 mg/mL, clear

actividad extraña

isocitrate dehydrogenase and aconitase ≤0.01%
malic dehydrogenase ≤0.1%

temp. de almacenamiento

2-8°C

Aplicación

Citrate Synthase from porcine heart has been used:
  • to inject newt egg for determining its importance in egg activation at fertilization
  • in the preparation of reaction mix to determine pyruvate carboxylase enzyme activity
  • to examine whether it can induce a [Ca2+] increase and egg activation in unfertilized eggs

Acciones bioquímicas o fisiológicas

Citrate synthase catalyses the conversion of citrate to acetyl-CoA in the presence of coenzyme-A with the release of H2O and oxaloacetate. The enzyme has a molecular weight of 85 kDa and a pI of 6.1-6.6. It is inhibited by fluoroacetyl-CoA, palmitoyl-CoA, and citroyl-CoA. It is also inhibited when it is acetylated by acetic anhydride or iodinated by iodine.

Definición de unidad

One unit will form 1.0 μmole of citrate from oxalacetate and acetyl CoA per min at pH 8.0 at 37 °C.

Forma física

Suspension in 3.2 M (NH4)2SO4 solution, pH 7.0.

Nota de preparación

Dissolves in water to form a clear solution at 1 mg/mL concentration.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Listados normativos

Los listados normativos se proporcionan para los productos químicos principalmente. Para los productos no químicos sólo se puede proporcionar información limitada. Si no hay ninguna entrada, significa que ninguno de los componentes está en la lista. Es obligación del usuario garantizar el uso seguro y legal del producto.

EU REACH Annex XVII (Restriction List)

CAS No.

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Certificados de análisis (COA)

Lot/Batch Number
SLCC7842
SLBW4809
SLBS4413
SLBM7486V
SLBJ0729V

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