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B7151

Sigma-Aldrich

Anti-Mouse IgG (Fab specific)–Biotin antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.46
En este momento no podemos mostrarle ni los precios ni la disponibilidad

origen biológico

goat

conjugado

biotin conjugate

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

secondary antibodies

clon

polyclonal

Formulario

buffered aqueous solution

técnicas

direct ELISA: 1:200,000-1:300,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
western blot: 1:400,000-1:800,000 using total cell extract of HeLa cells

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

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Descripción general

IgG (immunoglobulin G) antibody subtype is a predominant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. IgG is found in four subclasses IgG1, IgG2, IgG3 and IgG4. IgG has a monomeric structure.
The product binds to all mouse Igs

Aplicación

Anti-Mouse IgG (Fab specific)-Biotin antibody produced in goat was used in immunohistochemistry of lateral and medial menisci of rabbits and human adipose-derived stem cells.

Acciones bioquímicas o fisiológicas

IgG (immunoglobulin G) antibody participates in complement fixation and opsonization.
IgG antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to Anti-Mouse IgG (Fab specific) antibody allows for the binding of various labels such as avidin or streptavidin.

Otras notas

Antibody adsorbed with bovine, equine and human serum proteins.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Nota de preparación

Adsorbed to reduce background staining with bovine, horse or human samples.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Franklin T Moutos et al.
Tissue engineering. Part A, 16(4), 1291-1301 (2009-11-12)
Articular cartilage possesses complex mechanical properties that provide healthy joints the ability to bear repeated loads and maintain smooth articulating surfaces over an entire lifetime. In this study, we utilized a fiber-reinforced composite scaffold designed to mimic the anisotropic, nonlinear
Brian O Diekman et al.
Journal of biomedical materials research. Part A, 93(3), 994-1003 (2009-09-02)
Adipose-derived stem cells (ASCs) are multipotent progenitors that can be chondrogenically induced by growth factors such as bone morphogenetic protein 6 (BMP-6). We hypothesized that nonviral transfection of a BMP-6 construct (pcDNA3-BMP6) would induce chondrogenic differentiation of ASCs encapsulated in
Joseph F Poduslo et al.
Journal of neurochemistry, 102(2), 420-433 (2007-06-29)
Targeting therapeutic or diagnostic proteins to the nervous system is limited by the presence of the blood-brain barrier. We report that a F(ab')(2) fragment of a monoclonal antibody against fibrillar human Abeta42 that is polyamine (p)-modified has increased permeability at
Lisa J Kjonigsen et al.
Frontiers in neuroinformatics, 5, 2-2 (2011-04-27)
The rat hippocampal region is frequently studied in relation to learning and memory processes and brain diseases. The region is complex, consisting of multiple subdivisions that are challenging to delineate anatomically. Published atlases of the rat brain typically lack the
Nai-Chen Cheng et al.
Regenerative medicine, 6(1), 81-93 (2010-12-24)
To investigate the cell growth, matrix accumulation and mechanical properties of neocartilage formed by human or porcine articular chondrocytes on a porous, porcine cartilage-derived matrix (CDM) for use in cartilage tissue engineering. We examined the physical properties, cell infiltration and

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