E2886
ExtrAvidin®−Peroxidase
buffered aqueous solution
Sinónimos:
ExtrAvidin HRP
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About This Item
Productos recomendados
conjugado
peroxidase conjugate
formulario
buffered aqueous solution
técnicas
direct ELISA: 1:2,000
dot blot: 1:4,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
Condiciones de envío
wet ice
temp. de almacenamiento
2-8°C
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Especificidad
The product is found to be reactive with Anti-Avidin, Catalog Number A5170, and non-reactive with Anti-Streptavidin, Catalog Number S6390, by immunoelectrophoresis.
Aplicación
ExtrAvidin®-Peroxidase is suitable for the following applications:
- Dot blot at a minimum dilution of 1:4,000
- Immunohistochemistry (using formalin-fixed, paraffin-embedded sections at a dilution of 1:100)
- Direct ELISA at a minimum dilution of 1:2,000
- Immunoprecipitation and Immunobloting
- Ra2I solid-phase binding assays
- Southwestern blotting
- Western blotting
- Immunocytochemistry
Acciones bioquímicas o fisiológicas
Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin® is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin-Peroxidase may be used in conjunction with biotin in different techniques.
Forma física
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT
Nota de preparación
Affinity purified protein
Almacenamiento y estabilidad
Store at 2-8 °C. Do Not Freeze. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Información legal
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Skin Sens. 1
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Protocolos
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
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