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A9469

Sigma-Aldrich

ANTI-FLAG® M2 monoclonal antibody produced in mouse

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

Sinónimos:

Anti-ddddk, Anti-dykddddk

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About This Item

Código UNSPSC:
41106514
NACRES:
NA.32

origen biológico

mouse

conjugado

alkaline phosphatase conjugate

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

M2, monoclonal

formulario

buffered aqueous glycerol solution

reactividad de especies

all

concentración

~1 mg/mL

técnicas

indirect ELISA: 1:20,000

isotipo

IgG1

secuencia del inmunógeno

DYKDDDDK

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

Información sobre el gen

human ... ALPL(249)

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Descripción general

Monoclonal ANTI-FLAG® M2-Alkaline Phosphatase is a purified IgG 1 mouse antibody covalently conjugated to calf intestinal alkaline phosphatase (AP). The antibody conjugate binds to FLAG® fusion proteins and will recognize the FLAG® epitope at any position in the fusion protein (N-terminal, Met-N-terminal, C-terminal or internal FLAG® peptides).

Aplicación

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Monoclonal ANTI-FLAG® M2-Alkaline Phosphatase antibody produced in mouse has been used:
  • in direct tissue blot immunoassay of sweet orange petioles samples
  • in screening internalization of delta opioid receptor
  • for screening cell-free protein expression using ELISA

Forma física

Solution in Tris buffered saline containing 50% glycerol plus stabilizer and preservative

Información legal

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Waithaka Mwangi et al.
Journal of leukocyte biology, 78(2), 401-411 (2005-04-29)
Induction of immune responses against microbial antigens using DNA is an attractive strategy to mimic the immunity induced by live vaccines. Although DNA vaccines are efficacious in murine models, the requirement for multiple immunizations using high doses in outbred animals
Integration of cell-free protein coexpression with an enzyme-linked immunosorbent assay enables rapid analysis of protein-protein interactions directly from DNA
Layton CJ and Helling HW
Protein Science, 20(8), 1432-1438 (2011)
Matthew T Doherty et al.
The Journal of biological chemistry, 287(47), 39369-39379 (2012-10-06)
Myb repeats ∼52 amino acid residues in length were first characterized in the oncogenic Myb transcription factor, which contains three tandem Myb repeats in its DNA-binding domain. Proteins of this family normally contain either one, two, or three tandem Myb
Beth A Rasala et al.
Plant biotechnology journal, 9(6), 674-683 (2011-05-04)
Microalgae have the potential to be a valuable biotechnological platform for the production of recombinant proteins. However, because of the complex regulatory network that tightly controls chloroplast gene expression, heterologous protein accumulation in a wild-type, photosynthetic-competent algal chloroplast remains low.
Seongjoon Kang et al.
Biology, 7(2) (2018-05-09)
Chlamydomonas reinhardtii (Chlamydomonas) strains that are toxic to mosquito larvae because they express chloroplast transgenes that are based on the mosquitocidal proteins of Bacillus thuringiensis subsp. israelensis (Bti) could be very useful in mosquito control. Chlamydomonas has several advantages for
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