A7419
Avidin–Peroxidase
buffered aqueous solution
Sinónimos:
Avidin–HRP
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2 ML
841,00 €
841,00 €
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2 ML
841,00 €
About This Item
841,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Solicitar un pedido a granel
Productos recomendados
origen biológico
avidin from egg white
enzyme from horseradish
Nivel de calidad
conjugado
peroxidase conjugate
Formulario
buffered aqueous solution
técnicas
indirect ELISA: 1:100,000
western blot: 1:400,000-1:800,000 using detecting β-Actin in total cell extract of HeLa cells (5-10 μg/well)
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
Descripción general
Avidin is homotetrameric protein (68kDa) obtained from egg whites and binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues[1]. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.
Aplicación
Avidin-Peroxidase has been used for ELISA applications[2][3]. The product can also be used for western blot applications at dilutions ranging from 1:400,000 to 1:800,000.
Western blot analysis of proteins in HUVEC cells that had underwent cell surface biotinylation were detected using HRP-conjugated avidin at 33.3 ng/ml. [4]
Forma física
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.
Nota de preparación
Affinity purified protein.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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