83330
Pyruvate Kinase from rabbit muscle
crystalline suspension in 3.8 M ammonium sulfate solution, ~350 U/mg protein (10-40 mg/ml)
Sinónimos:
ATP:pyruvate 2-O-phosphotransferase, PK
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About This Item
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Formulario
suspension
calidad
crystalline suspension in 3.8 M ammonium sulfate solution
actividad específica
~350 U/mg protein (10-40 mg/ml)
mol peso
Mr ~230000
pH
~6
actividad extraña
lactate dehydrogenase ≤0.01%
temp. de almacenamiento
2-8°C
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Acciones bioquímicas o fisiológicas
Molecular Weight: 237 kDa and exists as a tetramer of four equal subunits of molecular weight 57 kDa.
Isoelectric Point: 7.6
Optimal pH: ∼7.5
Optimal Temperature: 25°C
ΕA280 = 0.54 for 1 mg(p)/ml, 1 cm path
Reported KM values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.
Isoelectric Point: 7.6
Optimal pH: ∼7.5
Optimal Temperature: 25°C
ΕA280 = 0.54 for 1 mg(p)/ml, 1 cm path
Reported KM values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.
Pyruvate kinase catalyzes the conversion of phosphoenolpyruvate to pyruvate, whitch is the rate-limiting final step in glycolysis.
Definición de unidad
1 U corresponds to the amount of enzyme which catalyzes the transphosphorylation of 1 μmol PEP per min at pH 7.4 and 25 °C
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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