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38185

Quantification kit for oxidized and reduced glutathione

200 tests

Sinónimos:

γ-L-glutamyl-L-cysteinylglycine kit, Quantification kit

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1 kit
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909,00 €

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NACRES:
NA.32
UNSPSC Code:
12352108

909,00 €


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Quality Segment

quality

200 tests

technique(s)

protein detection: suitable

storage temp.

2-8°C

General description

Glutathione Quantification Kit is designed to detect oxidized and reduced Glutathione. Glutathione is usually represented in a reduced form called GSH; however, GSH is converted into its oxidized state (GSSG) upon oxidative stress. The ratio between GSH and GSSG is considered an index for oxidative stress. Glutathione, also known as γ-L-glutamyl-L-cysteinylglycine, is a tripeptide compound that is involved in anti-oxidation and drug metabolism. It acts as a substrate for enzymes like glutathione peroxidase, glutathione S-transferase, and thiol transferase.

Application

Glutathione Quantification Kit is used to detect the total amount of glutathione as an index for oxidative stress or disease risk.

Features and Benefits

The detection ranges of total glutathione and GSSG using in this kit are from 0.5 μmol/l to 50 μmol/l and from 0.5 μmol/l to 25 μmol/l, respectively.

Analysis Note

Glutathione Quantification Kit contains a GSH masking reagent. This enables GSSG detection in the sample by measuring the absorption (λmax = 412 nm) obtained from a colorimetric reaction of DTNB (5,5’-dithiobis (2-nitrobenzoic acid)) coupled with the enzymatic recycling system. The total quantity of GSH can be measured by subtracting the amount of GSSG from the total amount of glutathione.

Other Notes

Glutathione Quantification Kit contains:
  • Enzyme Solution (50µl X 1)
  • Buffer Solution (60ml X 1)
  • Standard GSH (X 1)
  • Masking Reagent (20µl X 1)
  • Co-enzyme (X 2)
  • Substrate (DTNB) (X4)
  • Standard GSSG (X 1)
not available in Korea, Singapore, Taiwan and Japan

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Este artículo
CGP1GRSAG6013
description

200 tests

description

Sufficient for 100 colorimetric tests

description

Sufficient for 100 colorimetric tests

description

suitable for cell culture, BioReagent, ≥98.0%, powder

quality

200 tests

quality

-

quality

-

quality

-

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

300

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C


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Danger

Códigos de peligro

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - Skin Sens. 1

Clase de almacenamiento

3 - Flammable liquids

¿Qué está pasando?

WGK 3



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