If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
CGP1
Glutathione Peroxidase Cellular Activity Assay Kit
Sufficient for 100 colorimetric tests
Sinónimos:
Cellular glutathione peroxidase, c-GPx
Seleccione un Tamaño
| A ustedes/SKU | Disponibilidad | Precio |
|---|---|---|
1 kit | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 991,00 € |
Acerca de este artículo
991,00 €
Quality Segment
usage
sufficient for 100 tests
shelf life
Kit is stable for 24 months when unopened.
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
General description
Application
Biochem/physiol Actions
Other Notes
1 of 1
Este artículo | |||
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| usage sufficient for 100 tests | usage sufficient for 700 assays | usage sufficient for 100 tests | usage - |
| detection method colorimetric | detection method - | detection method colorimetric | detection method - |
| shipped in wet ice | shipped in wet ice | shipped in - | shipped in dry ice |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| shelf life Kit is stable for 24 months when unopened. | shelf life - | shelf life - | shelf life - |
Solo componentes del kit
- tert-Butyl hydroperoxide 1 mL
signalword
Danger
Hazard Classifications
Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Org. Perox. F - Skin Corr. 1C - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
Clase de almacenamiento
5.2 - Organic peroxides and self-reacting hazardous materials
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How can I determine the shelf life / expiration / retest date of this product?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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"Does A340/MIN refer to the absorbance value per minute, or is it the rate of decrease per minute?"
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The CGP1 assay measures the decrease in NADPH absorbance at 340 nm during the oxidation of NADPH to NADP+. This is a kinetic assay and the A340/MIN means absorbance per minute. Please see step 4 on page 4 of the technical bulletin for the kinetic program instructions. The number of readings being 6 for an interval time of 10 seconds which means data is for a minute. NADP+ does not have any absorbance at 340 nm and NADPH does. Thus, the absorbance will decrease in a sample with glutathione peroxide as all substrates, cofactors, enzymes are present for this conversion to occur.
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Can Product CGP1, Glutathione Peroxidase Cellular Activity Assay Kit, be used with Serum samples?
1 respuesta-
The Glutathione Peroxidase Cellular Activity Assay Kit (Product number: CGP1) is designed to measure Glutathione Peroxidase activity in cell lysates and tissue homogenates. This kit has not been validated for use with serum samples.
Please see product MAK437, Glutathione Peroxidase Assay Kit, which can be used for serum samples:
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Can Product CGP1, Glutathione Peroxidase Cellular Activity Assay Kit, be run in a 96 well plate?
1 respuesta-
Yes, but you would need to have a plate reader that has a kinetic function as this is a kinetic assay.
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What type of readings do I get with Product CGP1, Glutathione Peroxidase Cellular Activity Assay Kit?
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There is a decrease in absorbance during the assay. The OD values decrease with the increase in reaction time. The slope of the reduction in OD (delta OD per minute) is the value used to determine the enzyme units.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What dilution should I use for my sample when using Product CGP1, Glutathione Peroxidase Cellular Activity Assay Kit?
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In general, when human erythrocytes were assayed for the enzyme, the sample was diluted 10-fold (15 mg peroxidase per mL) and a 20 μl sample gave a decrease of 0.032 OD340 per minute. Too much enzyme will underestimate the apparent activity of the sample. When rabbit reticulocytes were assayed at a 10-fold dilution (10 mg peroxidase per mL), 20 μl gave a decrease of 0.065 OD340 per minute.
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How should I prepare my sample for analysis with Product CGP1, Glutathione Peroxidase Cellular Activity Assay Kit?
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The cells must be lysed before assaying with this kit. The best way to perform lysis is to incubate the cells with hypotonic buffer (at least 2 volumes and better with 3-4 volumes, depending on the expected level of peroxidase in these cells). Let them swell and homogenize with a Dounce homogenizer. Centrifuge the homogenate at 10,000 × g and use the supernatant for the assay. If a detergent is used for lysis, only use a peroxide-free detergent and follow the instructions in the Bulletin: "Nonionic detergents such as TWEEN and TRITON X-100 that contain high levels of endogenous peroxides will raise the apparent activity. If these detergents are vital to the extraction of the proteins of interest, a low peroxide detergent should be used such as Product Codes X-100-PC (TRITON X-100), P 6585 (TWEEN 20), or P 8192 (TWEEN 80)".
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Can Product CGP1, Glutathione Peroxidase Cellular Activity Assay Kit, be used with tissue samples?
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We have not tested the CGP1 assay with tissue samples. However, we suggest that the customer perform a small-scale pilot experiment using our CelLytic-MT lysis buffer (Product No. C3228). The buffer enables efficient extraction of tissue proteins. It does not contain any of the interfering agents that are listed on the kit bulletin.
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