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Merck

11966006001

Roche

TUNEL Dilution Buffer

pH 7.2-7.4 (20 °C), suitable for detection, pkg of 2 × 10 mL

Sinónimos:

TUNEL, transferase dUTP nick end labeling

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About This Item

Código UNSPSC:
41116100

envase

pkg of 2 × 10 mL

fabricante / nombre comercial

Roche

pH

7.2-7.4 (20 °C)

aplicaciones

detection

temp. de almacenamiento

2-8°C

Descripción general

The TUNEL dilution buffer can be used in combination with the in-situ cell death detection kits. It can be used to dilute terminal deoxynucleotidyl transferase (TdT) for the TUNEL reaction in special applications and also to label DNA strand breaks for detecting and quantitating apoptotic cell death at the single-cell level in cells and tissues.

Aplicación

TUNEL Dilution Buffer has been used:
  • to dilute terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) enzyme (TdT) in preparing TUNEL reaction mixture for TUNEL critical commercial assays[1]
  • to quantify apoptosis in rat kidney[2]
  • to dilute the terminal deoxynucleotidyl transferase enzyme solution to identify DNA fragmentation in oligodendrocytes in vitro[3]
  • for TUNEL assays[4]

TUNEL dilution buffer has been used in terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays.[5]

Nota de preparación

Working concentration: Working concentration of conjugate depends on application and substrate.


The following concentrations should be taken as a guideline:
  • ELISA: 100 mU/ml
  • Western blot: 100mU/ml


Storage conditions (working solution): Dilutions of TdT should be prepared immediately before use and should not be stored.
Keep TUNEL reaction mixture on ice until use.

Otras notas

For life science research only. Not for use in diagnostic procedures.

Pictogramas

Health hazard

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Aquatic Chronic 3 - Carc. 1B Inhalation

Código de clase de almacenamiento

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

does not flash

Punto de inflamabilidad (°C)

does not flash


Listados normativos

Los listados normativos se proporcionan para los productos químicos principalmente. Para los productos no químicos sólo se puede proporcionar información limitada. Si no hay ninguna entrada, significa que ninguno de los componentes está en la lista. Es obligación del usuario garantizar el uso seguro y legal del producto.

EU REACH Annex XVII (Restriction List)


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