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Merck

11089161001

Roche

Streptavidin-AP conjugate

solution, pkg of 1 mL (1,000U)

Sinónimos:

streptavidin

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About This Item

Código UNSPSC:
12352200

526,00 €


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Formulario

solution

Nivel de calidad

envase

pkg of 1 mL (1,000U)

fabricante / nombre comercial

Roche

temp. de almacenamiento

2-8°C

Descripción general

Streptavidin is covalently coupled to alkaline phosphatase (AP) for the preparation of streptavidin-AP-conjugate.

Alkaline Phosphatase
Alkaline Phosphatase is often used as a label in enzyme immunoassays. AP converts the sensitive substrate X-phosphate/NBT into a stable, water soluble product.

Aplicación

Streptavidin-AP has been used to detect biotinylated compounds using:
  • ELISA[1]
  • Immunohistocytochemistry
  • Western blot[2][3]

Forma física

Solution, stablilized in 50 mM Triethanolamine buffer, 3 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2, 10 mg/ml bovine serum albumin, pH 7.6.

Nota de preparación

Working solution: 50 - 1,000 mU/ml (i.e., stock solution can be diluted). Dilute the conjugate stock solution in 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.

Working concentration: Working concentration of conjugate will depend on the application and substrate. Indicated concentrations should be taken as a guideline.

ELISA: 50 - 200 mU/ml (1:5000 - 1:20,000 dilution)
Immunohisto/cytochemistry: 250 - 1,000 mU/ml (1:1000 - 1:4000 dilution)
Western blot: 200 - 500 mU/ml (1:2000 - 1:5000 dilution)

Stability: The concentrated stock solution is stable when stored at 2 to 8°C until the expiration date printed on the label. Do not freeze!

Otras notas

For life science research only. Not for use in diagnostic procedures.

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Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Skin Sens. 1

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

No data available

Punto de inflamabilidad (°C)

No data available


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