MABT524

Anti-Cortactin (p80/85) Antibody, clone 5G3.2

clone 5G3.2, from mouse

• Sinónimos: Src substrate cortactin, Amplaxin, Oncogene EMS1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 5G3.2, monoclonal
• reactividad de especies: mouse
• reactividad de especies (predicha por homología): human (based on 100% sequence homology)
• técnicas: western blot: suitable
• isotipo: IgG2bκ
• Nº de acceso NCBI: NP_005222
• Nº de acceso UniProt: Q14247
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CTTN(2017)

Descripción

Descripción general

Src substrate cortactin (UniProt Q14247; also known as Amplaxin, Oncogene EMS1) is encoded by the CTTN (also known as EMS1) gene (Gene ID 2017) in human. Cortactin is a cytosolic protein that promotes polymerization and rearrangement of the actin cytoskeleton, especially the actin cortex around the cellular periphery. Cortactin is activated via phosphorylation by upstream kinases in response to extracellular stimuli. Src is known to phosphorylate cortactin on Tyr421, Tyr466, and Tyr482, leading to cortactin interaction with F-actin and Arp2/3. Erk can also phosphorylate cortactin on Ser405 and Ser418, causing recruitment of additional actin nucleation-promoting factors (NPFs), most importantly N-WASP, to the Arp2/3/ complex. Phosphorylated cortactin thus recruits and stabilizes Arp2/3 complex on preexisting F-actin, providing a nucleation site for a new actin branch to form. Cortactin overexpression in tumor cells enhances invasiveness as a result of highly active lamellipodia formation, also known as invadopodia. Cortactin contains an N-terminal aspartic acid-aspartic acid-tryptophan (DDW) sequence (a.a. 29-31 in chicken; a.a. 20-22 in human & mouse) that mediates its interaction with Arp2/3, followed by six and a half repeat sequences (a.a. 80-125, 126-162, 163-199, 200-236, 237-273, 274-310, 311-333 in chicken; a.a. 80-116, 117-153, 154-190, 191-227, 228-264, 265-301, 302-324 in human and mouse) that mediates F-actin interaction, a proline-rich coiled coil domain (a.a. 349-410 in chicken; a.a. 348-401 in human and mouse) that mediates Src SH3 domain interaction, and a C-terminal SH3 domain (a.a. 505-563 in chicken; a.a. 492-550 in human a.a. 488-546 in mouse) that contains Erk phosphorylation sites and is important for cortactin interaction with NPFs other than Arp2/3.

Especificidad

Clone 5G3.2 epitope lies within the region containing cortactin repeats 3-6. Reactivity toward human spliced isoforms 2&3 that lack cortactin repeat 6 is possible, but has not been confirmed.

Inmunógeno

GST-tagged recombinant human cortactin fragment comprising cortactin repeats 3-6.

Aplicación

Research Category
Cell Structure

This mouse monoclonal Anti-Cortactin (p80/85), clone 5G3.2 Antibody, Cat. No. MABT524 is validated for use in Western Blotting for the detection of Cortactin.

Calidad

Evaluated by Western Blotting in 3T3/A31 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected cortactin in 10 µg of 3T3/A31 cell lysate.

Descripción de destino

~75 kDa observed. Target band size appears larger than the calculated molecular weights of 61.59/70.96/57.47 kDa (human isoform 1/2/3) and 61.25 kDa (mouse) due to posttranslational modification. Uncharacterized bands may be observed in some lysate(s).

Forma física

Format: Purified

Protein G purified.

Purified mouse IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable