MABD85

Anti-LIMD1 Antibody, clone 3F2/C6

clone 3F2/C6, from mouse

• Sinónimos: LIM domain-containing protein 1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 3F2/C6, monoclonal
• reactividad de especies: human
• técnicas: immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_055055
• Nº de acceso UniProt: Q9UGP4
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... LIMD1(8994)

Descripción

Descripción general

LIMD1 (LIM domains-containing protein 1) is a tumor suppressor and member of the Ajuba family of LIM domain-containing proteins. LIMD1 interacts with retinoblastoma protein (pRB), inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. Recently, LIMD1 has been implicated in regulating cellular processes involved in hypoxia. LIMD1 acts as a molecular scaffold, simultaneously binding the prolyl hydroxylases (PHDs) and von Hippel Lindau tumor suppressors (VHL), thereby assembling a PHD-LIMD1-VHL protein complex and creating an enzymatic niche that enables efficient degradation of HIF-1α.

Especificidad

This antibody recognizes human LIMD1.

Inmunógeno

Recombinant protein corresponding to human LIMD1

Aplicación

Detect LIMD1 using this Anti-LIMD1 Antibody, clone 3F2/C6 validated for use in ICC, IHC, Immunoprecipitation, Western Blotting.

Research Category
Apoptosis & Cancer

Stem Cell Research

Research Sub Category
Oncoproteins

Oxidative Stress

This antibody has been shown to work in Immunocytochemical applications (Foxler, D., et al. (2012).
This antibody has been shown to work in Immunohistochemical applications (Sharp, T., et al. (2008).
This antibody has been shown to work in Immunoprecipitation applications (Courtesy of Dr. Tyson Sharp, Cancer Research, UK)

Calidad

Evaluated by Western Blot in H1299 cell lysates.
Western Blot Analysis: A 1:2000 dilution of this antibody detected LIMD1 in H1299 cell lysates.

Descripción de destino

~72 kDa observed

Forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
H1299 cell lysates.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable