QPBCA

QuantiPro^™ BCA Assay Kit

for 0.5-30 μg/ml protein

• Sinónimos: BCA Assay, Protein determination by BCA, bicinchoninic acid reagent
• Código UNSPSC: 12161503
• NACRES: NA.84

Propiedades

• Nivel de calidad: 200
• Condiciones de envío: wet ice
• temp. de almacenamiento: 2-8°C

Descripción

Aplicación

In addition to protein determination and quantification in solution,[1][2][3] the QuantiPro^™ BCA Assay Kit has other applications, including determination of protein covalently bound to agarose supports and protein adsorbed to multiwell plates. It has also been applied to the determination of functional groups such as sulfhydryl, N-hydroxysuccinimido carboxylate, aldehyde and hydrazide on a variety of solid supports.

The kit can be used to measure very dilute protein concentrations in very small sample volumes. Accurately measures protein concentrations from 0.5 to 30 μg/mL in tube assays and 1 to 20 μg/mL in 96- or 384-well plate assays.

Características y beneficios

• Accurate across a broad range of protein concentrations
• High sensitivity; linear response form 0.5 to 30 μg/ml of protein
• Stable color complex
• Reduced susceptibility to detergents

Principio

Based on the alkaline reduction of Cu²⁺ to Cu¹⁺ by proteins, and the formation of a bicinchoninic acid:Cu¹⁺ complex having an absorbance maximum at 562 nm.

Almacenamiento y estabilidad

Store Reagents QA, QB, and QC at room temperature. Reagents QA and QB can be mixed together without Reagent QC (4% Copper(II) Sulfate Pentahydrate Solution 12 mL) and stored in a closed container at room temperature for a week. If this kit is received or stored cold, a precipitate may form in Reagent QA or Reagent QB. To dissolve the precipitate, warm the solution slowly to room temperature while mixing. The solutions are now suitable for use.

Información legal

QuantiPro is a trademark of Sigma-Aldrich Co. LLC

Solo componentes del kit

• Copper(II) sulfate solution, 4 % (w/v) (prepared from copper (II) sulfate pentahydrate) 12 mL

Los componentes del kit también están disponibles por separado

• P0914 Protein Standard Solution: 1.0 mg/ml bovine serum albumin in 0.15 M NaCl with 0.05% sodium azide (flame-sealed glass ampules) 10 x 1

Información de seguridad

• Pictogramas: GHS05,GHS09
• Palabra de señalización: Danger
• Frases de peligro: H290,H315,H318,H410
• Consejos de prudencia: P234 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• Clasificaciones de peligro: Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Irrit. 2
• Código de clase de almacenamiento: 8A - Combustible corrosive hazardous materials