MAB3776

Anti-MDM2 Antibody, clone SMP14

clone SMP14, Chemicon^®, from mouse

• Sinónimos: Murine Double Minute 2
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: SMP14, monoclonal
• reactividad de especies: rat, human, mouse
• fabricante / nombre comercial: Chemicon^®
• técnicas: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable
• isotipo: IgG1
• Nº de acceso NCBI: NM_002392.2; NM_006878.2; NM_006879.2; NM_006881.2; NM_006882.2
• Nº de acceso UniProt: Q00987
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... MDM2(4193) ; mouse ... Mdm2(17246) ; rat ... Mdm2(314856)

Descripción

Descripción general

MDM2 is over-expressed in many tumors. Its principal function is the ubiquitination and degradation of p53 tumour suppressor protein. This monoclonal antibody also recognizes a peptide epitope around Thr-216 of murine MDM2 when Thr-216 is unphosphorylated (Zhang & Prives, 2001). SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.

Especificidad

Recognizes human MDM2.

Inmunógeno

Synthetic peptide corresponding to residues 154-167 (CSRPSTSSRRRAISE) of human MDM2 cross-linked to KLH using glutaldehyde.

Aplicación

Research Sub Category
Ubiquitin & Ubiquitin Metabolism

RNA Binding Protein (RBP)

Use Anti-MDM2 Antibody, clone SMP14 (Mouse Monoclonal Antibody) validated in IP, IHC, IHC(P) to detect MDM2 also known as Murine Double Minute 2.

Western blot

Immunohistochemistry (Frozen and Paraffin-embedded sections)

Immunoprecipitation

ELISA

Optimal working dilutions must be determined by end user.

Forma física

Format: Purified

Liquid in 0.02M phosphate buffer, 0.25M NaCl, pH 7.6, with 0.1% sodium azide

Nota de análisis

Control
POSITIVE CONTROL: Breast carcinoma

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable