MAB3238
Anti-Cytokeratin 19 Antibody, clone RCK108
clone RCK108, Chemicon®, from mouse
Sinónimos:
Anti-CK19, Anti-K19, Anti-K1CS
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
purified antibody
tipo de anticuerpo
primary antibodies
clon
RCK108, monoclonal
reactividad de especies
human
fabricante / nombre comercial
Chemicon®
técnicas
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotipo
IgG1κ
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
dry ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... KRT19(3880)
Descripción general
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight from between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8, The individual cytokeratin polypeptides are designated 1-20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies.
Cytokeratin 19 (molecular mass 40 kD) is a marker of simple epithelia. Cytokeratin 19 has been found in mesothelial and mesothelioma cells, and in ovarian cysts, cystadenomas, and ovarian carcinomas. It has been shown to be present in the basal layer of non-keratinizing stratified squamous epithelia such as the oral cavity and the ectocervix. Cytokeratin 19 has also been found in adenocarcinomas of the lung and in tumor cells of pulmonary metastases.
It has been reported that cytokeratin 19 can be found in the ductal cells of normal pancreas and in pancreas cancers. It was found that ductal cells of normal pancreas express a number of cytokeratins including cytokeratin 19, whereas acinar cells of normal pancreas also express numerous cytokeratins but not cytokeratin 19. Thus it is determined that pancreas cancers consistently express certain cytokeratins including cytokeratin 19, as do normal ductal cells, which indicates that pancreas cancers may originate from normal ductal cells.
Cytokeratin 19 (molecular mass 40 kD) is a marker of simple epithelia. Cytokeratin 19 has been found in mesothelial and mesothelioma cells, and in ovarian cysts, cystadenomas, and ovarian carcinomas. It has been shown to be present in the basal layer of non-keratinizing stratified squamous epithelia such as the oral cavity and the ectocervix. Cytokeratin 19 has also been found in adenocarcinomas of the lung and in tumor cells of pulmonary metastases.
It has been reported that cytokeratin 19 can be found in the ductal cells of normal pancreas and in pancreas cancers. It was found that ductal cells of normal pancreas express a number of cytokeratins including cytokeratin 19, whereas acinar cells of normal pancreas also express numerous cytokeratins but not cytokeratin 19. Thus it is determined that pancreas cancers consistently express certain cytokeratins including cytokeratin 19, as do normal ductal cells, which indicates that pancreas cancers may originate from normal ductal cells.
Especificidad
Cytokeratin 19. MAB3238 reacts with glandular epithelial cells and most carcinomas. Does not react with hepatocytes and hepatocarcinoma
Inmunógeno
Cytoskeletal preparation of T24 cells.
Aplicación
Anti-Cytokeratin 19 Antibody, clone RCK108 is an antibody against Cytokeratin 19 for use in FC, WB, IC, IH(P).
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytokeratins
Cytokeratins
Western blot: 1:100-1:1,000; Recognizes a 40kDa band in reduced westerns
Immunohistochemistry: 1:100-1:200 on frozen and paraffin embedded tissue (after treatment with pepsin or trypsin) sections.
Immunocytochemistry: 1:100-1:200
Flow cytometry: 1:100-1:200.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: 1:100-1:200 on frozen and paraffin embedded tissue (after treatment with pepsin or trypsin) sections.
Immunocytochemistry: 1:100-1:200
Flow cytometry: 1:100-1:200.
Optimal working dilutions must be determined by the end user.
Forma física
Format: Purified
Liquid in buffer with 0.1% sodium azide.
Almacenamiento y estabilidad
Maintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles.
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Opcional
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Testing null
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