The thickness range of IPFL00010 is 110-145 microns.
IPFL00010
Immobilon® -FL PVDF Membrane
1 roll, 27 cm x 3.75 m, 0.45 µm pore size, transfer membrane with low background fluorescence
Immobilon®-FL PVDF Membrane
Sinónimos:
Western blotting membrane, blotting membrane, transfer membrane
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UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
Material:
PVDF membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, fluorometric
Pore size:
0.45 μm pore size
Compatibility:
for use with All fluorescence dyes, for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
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Permítanos ayudarleNombre del producto
Membrana de PVDF Immobilon®-FL, 1 roll, 27 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.
material
PVDF membrane, plain filter, white filter
feature
hydrophobic
manufacturer/tradename
Immobilon®
technique(s)
dot blot: suitable, western blot: suitable
filter L × W
27 cm × 3.75 m
pore size
0.45 μm pore size
capacity
155 μg/cm2 adsorption capacity (insulin), 205 μg/cm2 adsorption capacity (BSA), 300 μg/cm2 adsorption capacity (goat IgG)
compatibility
for use with All fluorescence dyes, for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
detection method
chemiluminescent, colorimetric, fluorometric
shipped in
ambient
Quality Level
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Este artículo | IPFL20200 | IPFL10100 | IPFL00005 |
|---|---|---|---|
| pore size 0.45 μm pore size | pore size 0.45 μm pore size | pore size 0.45 μm pore size | pore size 0.45 μm pore size |
| material PVDF membrane, white filter, plain filter | material PVDF membrane, plain filter, white filter | material PVDF membrane, plain filter, white filter | material PVDF membrane, plain filter, white filter |
| Quality Level 400 | Quality Level 400 | Quality Level 400 | Quality Level 400 |
| detection method chemiluminescent, fluorometric, colorimetric | detection method chemiluminescent, colorimetric, fluorometric | detection method chemiluminescent, colorimetric, fluorometric | detection method chemiluminescent, colorimetric, fluorometric |
| compatibility for use with All fluorescence dyes, for use with CPTS, for use with Ponceau-S red, for use with Amido black, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Coomassie brilliant blue | compatibility for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red | compatibility for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red | compatibility for use with All fluorescence dyes, for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby |
| manufacturer/tradename Immobilon® | manufacturer/tradename Immobilon® | manufacturer/tradename Immobilon® | manufacturer/tradename Immobilon® |
General description
La membrana de transferencia Immobilon®-FL es una membrana microporosa de fluoruro de polivinilideno (PVDF) con un tamaño nominal de poro de 0,45 μm. Está diseñada para adsorber las proteínas transferidas desde una variedad de matrices de gel. La membrana de transferencia Immobilon®-FL es hidrófoba y ofrece una estructura de poro uniformemente controlada con una gran capacidad de adsorción para biomoléculas. La membrana ofrece una transferencia óptima para proteínas con pesos moleculares superiores a 20 000. La membrana Immobilon®-FL muestra una autofluorescencia muy baja en una amplia gama de longitudes de onda de excitación/emisión. Esta propiedad la hace ideal para aplicaciones de transferencia que implican inmunodetección por fluorescencia. La membrana Immobilon®-FL también puede utilizarse para la detección quimioluminiscente o cromógena convencional.
Features and Benefits
Membrana Immobilon-FL:
- La primera membrana de transferencia optimizada para aplicaciones de fluorescencia; un fondo extremadamente bajo mejora la sensibilidad de todos los protocolos de detección de fluorescencia
- Compatible con todas las sondas fluorescentes utilizadas habitualmente en una amplia gama de longitudes de onda de excitación/emisión
- Mejor para detecciones de multiplexado y quimiofluorescencia
- Compatible con los bloqueantes y amortiguadores convencionales
Legal Information
Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
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Protocolos
Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.
This page shows and discusses three protocols for stripping and reprobing a western blot membrane.
Protocole de préparation d'échantillons pour la lyse cellulaire et une bonne extraction des protéines à partir de cellules et de tissus en culture en vue d'un western blotting ultérieur.
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What is the thickness of this product?
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Is it possible to use Immobilon FL instead of Immobilon P for western blots?
1 respuesta-
Yes, Immobilon FL can be used as an alternative to Immobilon P for western blots.
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Is 0.22 µm PVDF membrane available for fluorescent detection in Western blotting?
1 respuesta-
Immobilon PSQ, with a pore size of 0.2 µm, is suitable for various detection methods including fluorescent, radioactive, chromogenic, and chemifluorescent detection. But when using Immobilon PSQ in Western Blotting, it is important to consider that there may be increased background noise, which might require more stringent wash steps to ensure accurate results.
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Filtros activos
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Great for western blots
Traducir con GoogleI have been using this product for close to two and half years now. It is a highly reliable PVDF roll for western blotting. I use it to transfer a wide variety of proteins and use HRP conjugated secondary antibodies for imaging bands. The results have always been sharp, reliable and most importantly, reproducible. I shall buy again when we run out.
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