AB356492M

Anti-Cas9 (Chicken Polyclonal)

from chicken

• Sinónimos: SaCas9, CRISPR-associated endonuclease Cas9
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: chicken
• Nivel de calidad: 100
• forma del anticuerpo: unpurified
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• reactividad de especies: bacteria, Staphylococcus aureus
• técnicas: immunofluorescence: suitable; western blot: suitable
• isotipo: IgY
• Nº de acceso UniProt: J7RUA5
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

CRISPR-associated endonuclease Cas9 (UniProt: J7RUA5; also known as SaCas9, Cas9) is encoded by the Cas9 gene in Streptococcus aureus. Cas9 belongs to subtype II-A subfamily with one HNH Cas9-type domain. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. Cas9 has a bi-lobed architecture with a recognition lobe (residues 41-425) and a discontinuous nuclease lobe (NUC, residues 1-40 and 453-1053); the crRNA-target DNA lies in a channel between the 2 lobes. The NUC lobe has 2 endonuclease domains. The discontinuous RuvC-like domain in NUC cleaves the target DNA non-complementary to crRNA while the HNH nuclease domain cleaves the target DNA complementary to crRNA. Cas9 serves as a RNA-guided DNA endonuclease that cleaves double-stranded DNA targets with a protospacer adjacent motif (PAM) and complementarity to the guide RNA. The homologous region of the Streptococcus pyogenes is not closely related in amino acid sequence in S. aureus, hence this antibody does not recognize that protein.

Especificidad

This chicken polyclonal antibody detects Cas9 from Staphylococcus aureus. It targets an epitope within 251 amino acids from the C-terminal end. This antibody does not react with Cas9 from Streptococcus pyogenes.

Inmunógeno

Epitope: C-terminus

His-tagged recombinant fragment corresponding to 251 amino acids from the C-terminal end of CRISPR-associated endonuclease Cas9 from Staphylococcus aureus.

Aplicación

Anti-Cas9 (Chicken Polyclonal), Cat. No. AB356492, is a highly specific chicken polyclonal antibody that targets CRISPR-associated endonuclease Cas9 and has been tested in Immunofluorescence and Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Cas9 in HEK293 cells transfceted with GFP-Cas9 from Staphylococcus aureus (Courtesy of Dr. Gerry Shaw).

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Cas9 in HEK293 cells transfceted with GFP-Cas9 from Staphylococcus aureus (Courtesy of Dr. Gerry Shaw).

Calidad

Evaluated by Western Blotting in HEK293 cells transfected with GFP-Cas9 from Staphylococcus aureus.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Cas9 in 40 µg of lysate from HEK293 cells transfected with GFP-Cas9 from Staphylococcus aureus.

Descripción de destino

~57 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Forma física

Chicken polyclonal antibody in PBS with 0.02% sodium azide.

Format: Unpurified

Unpurified

Almacenamiento y estabilidad

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable