648310-M

Tris(hidroximetil)-aminometano

≥99% (perchloric acid titration), solid, pH 7.0-9.0, solid, Molecular Biology Grade

• Sinónimos: 2-Amino-2-(hidroximetil)-1,3-propanodiol, Base tris, THAM, Trometamol
• Fórmula lineal: NH₂C(CH₂OH)₃
• Número de CAS: 77-86-1
• Peso molecular: 121.14
• Beilstein: 741883
• Número MDL: MFCD00004679
• Código UNSPSC: 12161705
• NACRES: NA.25

Propiedades

• Nombre del producto: Tris(hidroximetil)-aminometano, TRIS base is useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.
• grado: Molecular Biology
• Nivel de calidad: 100
• Ensayo: ≥99% (perchloric acid titration)
• Formulario: solid
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze
• color: white
• intervalo de pH útil: 7-9
• pKa (25 °C): 8.1
• bp: 219-220 °C/10 mmHg (lit.)
• mp: 167-172 °C (lit.)
• solubilidad: water: 500 mg/mL
• trazas de catión: heavy metals: ≤0.0005%
• Absorción: ≤0.02 at 280 nm; ≤0.03 at 260 nm
• actividad extraña: RNases, DNases, proteases, none detected
• temp. de almacenamiento: 10-30°C
• cadena SMILES: NC(CO)(CO)CO
• InChI: 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• Clave InChI: LENZDBCJOHFCAS-UHFFFAOYSA-N

Descripción

Descripción general

Useful in the pH range of 7.0-9.0. Has a pK_a of 8.1 at 25°C.

Widely used as a biological buffer (absorbance: ≤0.03 at 260 nm; ≤0.02 at 280 nm). Useful in the pH range of 7.0-9.0. Has a pK_a of 8.1 at 25°C.

Aplicación

• A Fast and Automated Melanin-bleaching Method for Histopathologic Evaluation of Pigmented Melanoma Tissues.: This study presents an automated melanin-bleaching technique using a Tris-based buffer to enhance histopathologic evaluation of pigmented melanoma tissues, aiding in more accurate diagnoses (Ugolini et al., 2022).


• Improvement of plant protein solubilization and 2-DE gel resolution through optimization of the concentration of Tris in the solubilization buffer.: Highlights the optimization of Tris concentration in solubilization buffers to enhance protein solubilization and gel electrophoresis resolution, crucial for biochemical studies (Cho et al., 2010).

Advertencia

Toxicity: Irritant (B)

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable