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69671

Millipore

Thrombin, Restriction Grade

Sinónimos:

Thrombin, Restriction Grade

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About This Item

Número de CAS:
9002-04-4
Número MDL:
MFCD18251359
Código UNSPSC:
12352202
NACRES:
NA.54

origen biológico

human plasma

Nivel de calidad

400

Formulario

liquid

fabricante / nombre comercial

Novagen®

condiciones de almacenamiento

OK to freeze
avoid repeated freeze/thaw cycles

técnicas

protein extraction: suitable

idoneidad

suitable for additive or modifier in the separation of proteins or peptides

aplicaciones

life science and biopharma

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

Descripción general

Restriction Grade Thrombin is a highly purified preparation qualified to specifically cleave target proteins produced with appropriate vectors. This preparation is functionally tested for activity with fusion proteins and is free of detectable contaminating proteases. Prepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV. Thrombin is supplied as a solution in 50% glycerol and includes 10X Thrombin Cleavage Buffer and a Cleavage Control Protein.

Componentes

•50 UThrombin

•1 ml10X Thrombin Cleavage Buffer

•2 ml1X Thrombin Dilution/Storage Buffer

•10 µgCleavage Control Protein

Advertencia

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Definición de unidad

One unit is defined as the amount of enzyme needed to cleave 1 mg of fusion protein in 16 hours at 20°C in a 200 µl reaction containing 20 mM Tris-HCl pH 8.4, 150 mM NaCl, 2.5 mM CaCl₂, 50 µg fusion protein.

Nota de preparación

Prepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Información legal

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Jooyoung Park et al.
eLife, 8 (2019-12-20)
The computational design of a symmetric protein homo-oligomer that binds a symmetry-matched small molecule larger than a metal ion has not yet been achieved. We used de novo protein design to create a homo-trimeric protein that binds the C3 symmetric
Christian M Smolko et al.
Scientific reports, 9(1), 19409-19409 (2019-12-21)
Protein kinases are enzymes whose abundance, protein-protein interactions, and posttranslational modifications together determine net signaling activity in cells. Large-scale data on cellular kinase activity are limited, because existing assays are cumbersome, poorly sensitive, low throughput, and restricted to measuring one

Artículos

Cleavage Enzymes for Fusion Protein Purification

Find highly-specific cleavage enzymes for isolating proteins during fusion protein purification including enterokinase, factor Xa, HRV 3C protease, and thrombin.

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