Prior to lyophilization the thrombin is at concentration of 1250-1450 units/mL in a solution of saline sodium citrate buffer, pH 6.5.
T7009
Trombina from human plasma
lyophilized powder, ≥1,000 NIH units/mg protein (E1%/280, 18.3)
Sinónimos:
Factor IIa
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| A ustedes/SKU | Disponibilidad | Precio |
|---|---|---|
100 units | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 139,00 € |
250 units | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 286,00 € |
1000 units | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 696,00 € |
5000 units | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 2850,00 € |
Acerca de este artículo
Número CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-648-7
MDL number:
Specific activity:
≥1,000 NIH units/mg protein (E1%/280, 18.3)
139,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
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Permítanos ayudarleGeneral description
The F2 gene encodes thrombin protein and is mapped to human chromosome 11p11.2.
Application
Thrombin from human plasma has been:
- dissolved in CaCl2 and added to poly ethylene glycolated fibrinogen/cell solution for fibrin production
- to polymerize fibrin on well plates
- used as an endogenous agonist proteinase for proteinase-activated receptors : PAR1, PAR3 and PAR4 for stimulation of cells
Biochem/physiol Actions
Serín proteasa que hidroliza selectivamente los enlaces Arg-Gly en el fibrinógeno para formar fibrina y fibrinopéptidos A y B.
Thrombin is an endolytic serine protease which stimulates vascular damage induced-leukocytes, platelets, endothelial and mesenchymal cell production. This action is mediated via G-protein-coupled receptor. It catalysis the cleavage of fibrinogen at Arg−Gly bonds to form fibrin and release fibrinopeptides A and B. The predominant form of thrombin in vivo is the zymogen prothrombin (factor II), which is produced in the liver. Prothrombin is a coagulation activation factor, high levels of which indicates cancer. Thrombin can be used to cleave fusion proteins. Cleavage of fusion proteins can be carried out at a thrombin:fusion protein ratio of 1:500.
Physical form
Lyophilized from saline sodium citrate buffer, pH 6.5
Analysis Note
The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.
Other Notes
Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard
View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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Este artículo | |||
|---|---|---|---|
| Gene Information human ... F2(2147) | Gene Information human ... F2(2147) | Gene Information human ... F2(2147) | Gene Information cow ... F2(280685) |
| specific activity ≥1,000 NIH units/mg protein (E1%/280, 18.3) | specific activity 1500-3500 NIH units/mg protein (E1%/280, 18.3) | specific activity - | specific activity 600-2,000 NIH units/mg protein (biuret) |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) - | application(s) diagnostic assay manufacturing |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
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signalword
Danger
Clase de almacenamiento
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
target_organs
Respiratory system
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
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Which concentration and buffer was t7009 human thrombin dissolved in when lyophillized?
1 respuesta-
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Hello! How long can we store the lyophilised powder in stock solutions at -20°C?
1 respuesta-
Stability of thrombin in solution is not evaluated. However, external reports have shown a 10% loss of activity per year when reconstituted at a concentration of 1mg/mL and stored at -20C. Freeze/thaw cycles should be avoided.
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Which volume of liquid do I have to add to a 100 U T7009 vial? And T6884 vial? With which kind of liquid should the powder be dissolved?
1 respuesta-
The product is soluble in water (10 mg/mL). Stock solutions can also be prepared at a concentration of 100 units/mL in a 0.1% (w/v) BSA solution. Stock solutions remain active for one week at 0–5 °C. Solutions are most stable at pH 6.5, as a pH >7 will greatly reduce thrombin activity. Since thrombin solutions adsorb to glass, it is recommended to aliquot the solutions in plastic tubes and store at –20 °C for long-term storage. We can calculate the weight in MG for this lot of 250 UN product using lot specific information found on a CoA (Refer to a certificate).
For T7009-250UN for example: As per one lot in one CoA, units/mg is 1742.
1742 units = 1 mg
250 units = 1mg/1742 x 250 = 0.1435 mg
So, 250 units of this product lot contains 0.1435 mg (143.5 ug) of powder in vial.¿Le ha resultado útil?
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For thrombin, how do NIH units compare to International Units (IU)?
1 respuesta-
One NIH Unit is equivalent to one International Unit (IU).
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How much is 100UN of thrombin(T6884)? what is the weight in mg?
1 respuesta-
Both of these Thrombin products - T7009 and T6884 - are sold by activity or units. The amount of powder in the vial is lot specific and based on the activity reported in the Certificate of Analysis.
T6884-100UN, lot SLCP0517 tested at 2736 uits per milligram. The 100UN vial contains 36ug of powder per vial. See the link below to review this Certificate of Analysis:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/T6884-100UNSLCP0517.pdfT7009-100UN, lot SLCN9908 tested at 1742 units per milligram. The 100UN vial contains 57ug of powder per vial. See the link below to review this Certificate of Analysis:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/T7009-250UNSLCN9908.pdf¿Le ha resultado útil?
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How much does the T-7009-100UN weigh with and without packaging? Country of origin Germany?
1 respuesta-
This product is packaged in units, the exact amount of product will depend on the lot-specific activity. For example, the SLCN9908 has a specific activity of 1742 units/ mg protein. The end user will receive 0.06 mg. The net weight of packaging is 1,000 mg. The product is manufactured in the USA.
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What is the Department of Transportation shipping information for this product?
1 respuesta-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the solubility of Product T7009, Thrombin from human plasma?
1 respuesta-
Thrombin from human plasma gave a clear very faint yellow solution when dissolved at 100 unit vial plus 1 ml of 28 mM sodium barbital, 0.125M sodium chloride, 1% BSA, 0.5% PEG, pH 7.35. Thrombin from bovine plasma (T4648) is soluble in water at 10 mg/mL.
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What is the isoelectric point of Product T7009, Thrombin from human plasma?
1 respuesta-
The isoelectric point (pI) range of the human thrombin isozymes is 6.35 - 7.6. The major isoform has a pI of 7.31.
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What is the cross reactivity of Thrombin?
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Thrombin from any vertebrate species will clot the fibrinogen of virtually any other species. Fibrinogen from any mammalian source will cross-react with thrombin from any mammalian source, but it is not well known how thrombin and fibrinogen from different animals differ in their sequence. When any mammalian thrombin is injected into a different mammal, clotting will occur.
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