Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~49 kDa JNK1/SAPK1 protein and the ~55 kDa JNK2/SAPK2 protein phosphorylated at Thr183 and Tyr185.
Recognizes the ~49 kDa SAPK1 protein and the ~55 kDa SAPK2 protein phosphorylated at Thr183 and Tyr185.
This PhosphoDetect Anti-SAPK/JNK (pThr¹⁸³, Tyr¹⁸⁵) Rabbit pAb is validated for use in WB, IHC for the detection of SAPK/JNK (pThr¹⁸³, Tyr¹⁸⁵).
Inmunógeno
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸³ and Tyr¹⁸⁵ phosphorylation sites of human SAPK/JNK
Aplicación
Immunoblotting (1:1000)
Immunohistochemistry (see comments)
Advertencia
Toxicity: Standard Handling (A)
Forma física
In Dulbecco′s PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.3.
Reconstitución
Following initial thaw, aliquot and freeze (-70°C).
Otras notas
Fanger, G.R., et al. 1999. Histol. Histopathol.14, 887. Gabai, V.L., et al. 1998. FEBS Lett.438, 1.
Specific for the dually phosphorylated form of JNK/SAPK containing a phosphate on threonine 183 and tyrosine 185. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the proper working dilution.
Información legal
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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