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Sigma-Aldrich

Ribonuclease Inhibitor, Human, Recombinant, E. coli

The Ribonuclease Inhibitor, Human, Recombinant, E. coli controls the biological activity of Ribonuclease. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.

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About This Item

Código UNSPSC:
12352200

Nivel de calidad

Formulario

liquid

actividad específica

≥20,000 units/mL

fabricante / nombre comercial

Calbiochem®

condiciones de almacenamiento

OK to freeze

actividad extraña

Endonuclease and RNase, none detected

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

Descripción general

Recombinant, human ribonuclease inhibitor expressed in E. coli. Non-competitive inhibitor that inactivates RNase by non-covalent binding. Has been used to improve cDNA synthesis and in vitro RNA synthesis, increase yields of polysomes and aid in the preparation of RNase-free antibodies. Inhibits RNases A, B, and C. Does not inhibit RNase T1 and S1 nuclease from Aspergillus.
Recombinant, human ribonuclease inhibitor expressed in E. coli. Non-competitive inhibitor that inactivates RNase by non-covalent binding. Has been used to improve cDNA synthesis and in vitro RNA synthesis, increase yields of polysomes, and aid in the preparation of RNase-free antibodies. Inhibits RNases A, B, and C. Does not inhibit RNase T1 and S1 nuclease from Aspergillus.

Acciones bioquímicas o fisiológicas

Cell permeable: no
Primary Target
inactivates RNase
Product does not compete with ATP.
Reversible: no

Advertencia

Toxicity: Standard Handling (A)

Definición de unidad

One unit is defined as the amount of material that will inhibit 50% of the activity of 5 ng of RNase A.

Forma física

In 50 mM KCl, 20 mM HEPES, 8 mM DTT, 50% glycerol.

Reconstitución

Maintain at -20°C. Avoid freezing solutions. Addition of DTT is recommended to maintain 8 mM level.

Otras notas

Saxena, S.K., et al. 1992. J. Biol. Chem. 267, 21982.
Lee, F.S., et al. 1989. Biochemistry 28, 225.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Nida Ali et al.
Nucleic acids research, 48(2), 847-861 (2019-12-06)
RNase E is a 472-kDa homo-tetrameric essential endoribonuclease involved in RNA processing and turnover in Escherichia coli. In its N-terminal half (NTH) is the catalytic active site, as also a substrate 5'-sensor pocket that renders enzyme activity maximal on 5'-monophosphorylated
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The Journal of general virology, 101(6), 676-684 (2020-05-08)
Rose rosette virus (RRV) (genus Emaravirus) is the causal agent of the homonymous disease, the most destructive malady of roses in the USA. Although the importance of the disease is recognized, little sequence information and no full genomes are available
Vera Thole et al.
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Flavonoids are produced in all flowering plants in a wide range of tissues including in berry fruits. These compounds are of considerable interest for their biological activities, health benefits and potential pharmacological applications. However, transcriptomic and genomic resources for wild
Claudia S Barros et al.
STAR protocols, 3(4), 101735-101735 (2022-10-02)
Here, we describe a protocol to remove single identified cells directly from Drosophila living brains and analyze their transcriptome. We detail the steps to harvest fluorescent cells using a capillary under epifluorescence and transmitted light to avoid contamination. We then
Ihshan Akthar et al.
Reproduction (Cambridge, England) (2019-12-04)
We previously reported that sperm binding to cultured monolayers of bovine uterine epithelial cells induces an acute inflammatory response involving the Toll-like receptor (TLR2) signaling pathway. This response serves to clear the uterus of sperm and thereby prepares the endometrium

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