492027-M

NRG1 Isoform SMDF, Human, Recombinant

• Sinónimos: Heregulin-1
• Código UNSPSC: 12352203

Propiedades

• Ensayo: >95% (SDS-PAGE)
• Nivel de calidad: 100
• Formulario: lyophilized
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze
• impurezas: ≤1.0 EU/μg protein Endotoxin
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Recombinant, human neuregulin 1 (NRG1), isoform SMDF (sensory and motor neuron-derived factor), consisting of amino acids 1-263, 117-296, and 162-296, each fused at the C-terminus to a His•Tag sequence and expressed in S. frugiperda Sf21 cells using a baculovirus expression system. The neuregulin (also known as heregulin) family of cytokines is comprised of multiple secreted or membrane-bound isorforms that are produced from the single heregulin gene by alternative splicing and/or usage of different promoters. NRG1 isoform SMDF is a type III isoform with a C-terminal EGF-like domain that interacts with the erbB family of tyrosine kinase receptors. NRG1 isoform SMDF expression has been found to be restricted to the nervous system and is believed to play an important role in the development of the nervous system.

Acciones bioquímicas o fisiológicas

ED₅₀ = 1-5 ng/ml as measured in a serum-free proliferation assay using MCF-7 human breast adenocarcinoma cells.

Advertencia

Toxicity: Standard Handling (A)

Forma física

Lyophilized from 0.2 µm filtered PBS, BSA.

Reconstitución

Following reconstituion refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 100 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.

Otras notas

Meyer, D. et al. 1997. Development124, 3575.
Yarden, Y. and D. Wen 1994. in Guidebook to Cytokinesand Their Receptors, N.A. Nicola, Ed., Oxford University Press, p. 146.
Karey, K.P., et al. 1988. Cancer Res.48, 4083.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3