431525

L-Leucine Dehydrogenase, Bacillus cereus

• Sinónimos: L-Leucine Dehydrogenase, Bacillus cereus, L-LeuDH, L-Leucine:NAD ⁺-oxidoreductase
• Número de CAS: 9082-71-7
• Comisión internacional de enzimas: 1.4.1.9 (BRENDA, IUBMB)
• Número MDL: MFCD00147461
• Código UNSPSC: 12352202
• NACRES: NA.77

Propiedades

• origen biológico: Bacillus sp. (Bacillus cereus)
• Nivel de calidad: 100
• formulario: liquid
• actividad específica: ≥10 units/mg protein; ≥50 units/mL
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; avoid repeated freeze/thaw cycles
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Native L-leucine dehydrogenase from Bacillus cereus. Catalyzes the NAD⁺-dependent conversion of L-leucine to α-ketoisocaproate. Suitable for the production of L-tertiary leucine amino acids.

Native L-leucine dehydrogenase from Bacillus cereus. Purified enzyme that catalyzes the NAD-dependent conversion of L-leucine to α-ketoisocaproate. Suitable for the production of L-tertiary leucine amino acids. Has an optimal pH of 10.5-10.8 for L-leucine substrate and 9.4 for α-ketoisocaproate substrate. Has an optimal temperature of 65°C.

pH optimum 10.5-10.8 (L-leucine substrate) and 9.4 (α-ketoisocaproate substrate), temperature optimum 65°C.

Advertencia

Toxicity: Standard Handling (A)

Definición de unidad

One unit is defined as the amount of enzyme that will convert 1.0 µmol L-leucine to α-ketoisocaproate in the presence of NAD⁺ per min at 30°C, pH 10.7.

Forma física

In 100 mM NaCl, 10 mM potassium phosphate buffer, 50% glycerol, pH 7.5.

Reconstitución

Following initial thaw, aliquot and freeze (-20°C).

Otras notas

Kärst, U., et al. 1986. FEMS Microbiol. Lett. 37, 335.
Schütte, H., et al. 1985. Appl. Microbiol.Biotechnol.22, 306.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2