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L6007

Leucine Aminopeptidase, microsomal from porcine kidney

Type VI-S, lyophilized powder, ≥12 units/mg protein (biuret)

Sinónimos:

Aminopeptidase M, Aminopeptidase N, Cathepsin III, LAP, Leucinamide aminopeptidase, Leucinaminopeptidase

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A ustedes/SKUDisponibilidadPrecio
25 units
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
509,00 €
50 units
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
758,00 €
100 units
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1400,00 €

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Número CAS:
9054-63-1
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-942-5
MDL number:
MFCD00131501
Número CE:
3.4.11.2 (BRENDA, IUBMB)
Specific activity:
≥12 units/mg protein (biuret)
Biological source:
Porcine kidney

509,00 €

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biological source

Porcine kidney

type

Type VI-S

form

lyophilized powder

specific activity

≥12 units/mg protein (biuret)

mol wt

320 kDa

composition

Protein, 30-70%

storage temp.

−20°C

General description

Leucine Aminopeptidase (LAP) from porcine is a 320-kDa exopeptidase present in cytosol. It contains zinc in its structure and belongs to the M1 and M17 peptidase families. LAP display bi-lobed structure and has the catalytic domain at the C-terminus and a variable N-terminal domain.

Application

Leucine Aminopeptidase, microsomal from porcine kidney has been used in aminopeptidase N (APN) inhibitor screening assay in adenocarcinoma cell line ES-2 and in the inhibition kinetic studies with APN inhibitor.[1]

Biochem/physiol Actions

Leucine Aminopeptidase (LAP) catalyzes protein hydrolysis at the leucine residue at N-terminal. An altered level of LAP is implicated in tumor proliferation and progression. LAP is a potential biomarker for ovarian epithelial cancer therapy. Porcine LAP hydrolyzes glycine and arginine substrates.

Physical form

Contains primarily phosphate buffer salts, pH 7.0

Other Notes

One unit will hydrolyze 1.0 μmole of L-leucine-p-nitroanilide to L-leucine and p-nitroaniline per min at pH 7.2 at 37 °C. (At 25 °C, approx. 40% of the activity at 37 °C is obtained.) The activity obtained using L-leucine p-nitroanilide as substrate is 2-5 times that obtained with L-leucinamide as substrate.

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Este artículo
L5006P6675164598
Leucine Aminopeptidase, microsomal from porcine kidney Type VI-S, lyophilized powder, ≥12 units/mg protein (biuret)

Sigma-Aldrich

L6007

Leucine Aminopeptidase, microsomal from porcine kidney

Leucine Aminopeptidase, microsomal from porcine kidney Type IV-S, ammonium sulfate suspension, 10-40 units/mg protein (Bradford)

Sigma-Aldrich

L5006

Leucine Aminopeptidase, microsomal from porcine kidney

Prolidase from porcine kidney lyophilized powder, ≥100 units/mg protein

Sigma-Aldrich

P6675

Prolidase from porcine kidney

Aminopeptidase M, Porcine Kidney

Sigma-Aldrich

164598

Aminopeptidase M, Porcine Kidney

specific activity

≥12 units/mg protein (biuret)

specific activity

10-40 units/mg protein (Bradford)

specific activity

≥100 units/mg protein

specific activity

-

biological source

Porcine kidney

biological source

-

biological source

-

biological source

Porcine kidney

form

lyophilized powder

form

ammonium sulfate suspension

form

lyophilized powder

form

suspension (crystalline)

mol wt

320 kDa

mol wt

-

mol wt

-

mol wt

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

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pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number

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Preguntas

1–2 de 2 Preguntas  

  1. I would like to know the optimum solvent for dissolving, storing and using this reagent (Cat # L6007-25UN).

    1 respuesta

    1. According to the Enzymatic Assay for Leucine Aminopeptidase, Microsomal Enzyme Solution, prepare a solution containing 0.10 - 0.15 unit/ml of Leucine Aminopeptidase, Microsomal in cold deionized water immediately before use.

      More information can be found at https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/356/218/l6007enz.pdf.

      This formulation contains phosphate buffer salts. For reconstitution, storage, and use of this product, 50 mM Sodium Phosphate Buffer, pH7.2, is a suitable solvent.

      ¿Le ha resultado útil?

  2. I recently purchased Leucine Aminopeptidase from porcine kidney Cat # L6007-25UN. I'd like to inquire how many mgs of protein is in one vial.

    1 respuesta

    1. The milligrams of protein per vial will depend on the units/mg protein defined on the product's certificate of analysis. For example, lot SLCM3898 reports specific activity of 19 units/mg protein. This results in approximately 1.3 mg of protein per vial for this particular lot. The protein content of this lot of material is reported at 61%, therefore the total mass of solid material in the 25UN vial will total around 2.2 mg.

      (Link to certificate of analysis: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/L6007-BULKSLCM3898.pdf)

      ¿Le ha resultado útil?

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